# US 9,872,927 B2 · Dye solution

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- Number: US 9,872,927 B2
- Document ID: US9872927B2
- Status: Granted
- Kind: B2
- Application number: 15/279,655
- Filed: 2016-09-29
- Published: 2017-01-19
- Granted: 2018-01-23
- Earliest claimed: 2008-12-19
- Assignees: Fluoron GmbH
- Applicants: Fluoron GmbH
- Inventors: Christian Lingenfelder; Bastian Theisinger; Wilfried Hiebl; Nadine Hagedorn
- Web page: https://patentgrep.com/patent/US9872927B2

## CPC

- A61K 49/006: {Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery}
- A61P 41/00: Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
- C09B 67/0083: {Solutions of dyes}
- C09B 67/0089: {non ionic dispersing agent, e.g. EO or PO addition products}
- G01N 1/30: Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis}
- A61F 9/00: Methods or devices for treatment of the eyes; Devices for putting in contact-lenses; Devices to correct squinting; Apparatus to guide the blind; Protective devices for the eyes, carried on the body or in the hand (caps with means for protecting the eyes A42B1/0181; visors for helmets A42B3/22; eye baths A61H35/02; sunglasses or goggles having the same features as spectacles G02C)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C09B: ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS}; MORDANTS; LAKES (fermentation or enzyme using processes to synthesise a desired chemical compound C12P)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C)

## Abstract

The invention relates to a water-based biocompatible non-cytotoxic preparation for the selective staining of internal limiting membrane (ILM) and/or epiretinal membranes (ERM) in the human or animal eye, and to a kit containing said water-based preparation according to the invention.

## Claims

### Claim 1

A method of staining and removing the internal limiting membrane (ILM) and/or of epiretinal membranes (ERM) in the human or animal eye, comprising
preparing a water-based, biocompatible eye membrane dye solution comprising at least one dye selected from the group consisting of triphenylmethane dyes, azo dyes, cyanine dyes, natural dyes, and mixtures thereof, wherein the eye membrane dye solution has a density in the range of 1.01 g/cm3 to 1.5 g/cm3, wherein the eye membrane dye solution has a dynamic viscosity in the range of 1 to 500 mPas at 25° C. and a shear rate of 10 s−1 with the proviso that monosaccharides or reducing disaccharides are not used for adjusting the density;
staining the ILM and/or of ERM by using the eye membrane dye solution; and
removing the stained ILM and/or ERM.

### Claim 2 (depends on 1)

The method of claim 1, further comprising a dye selected from the group consisting of azo dyes, cyanine dyes, natural dyes and mixtures thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein said at least one dye stains the internal limiting membrane or the epiretinal membrane so that the membranes are distinguished optically from the retina.

### Claim 4 (depends on 1)

The method of claim 1, wherein the eye membrane dye solution contains an agent for adjusting the density.

### Claim 5 (depends on 4)

The method of claim 4, wherein the agent for adjusting the density is heavy water.

### Claim 6 (depends on 4)

The method of claim 4, wherein the agent for adjusting the density is selected from the group consisting of polyether, polyvinyl alcohol, polyester, polyacrylic acid copolymer and polyvinyl pyrrolidone.

### Claim 7 (depends on 1)

The method of claim 1, wherein the dye is Brilliant Blue G.

### Claim 8 (depends on 7)

The method of claim 7, wherein the concentration of the Brilliant Blue G in the eye membrane dye solution is up to 0.3 g/l.

### Claim 9 (depends on 1)

The method of claim 1, wherein the eye membrane solution is used as a dye for a negative representation of epiretinal membranes.

### Claim 10 (depends on 1)

The method of claim 1, wherein the eye membrane dye solution has an osmolarity in the range of 280-330 mosmol/l.