# US 9,872,892 B2 · Animal feed enzymes

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- Number: US 9,872,892 B2
- Document ID: US9872892B2
- Status: Granted
- Kind: B2
- Application number: 14/779,121
- Filed: 2014-05-08
- Published: 2016-02-25
- Granted: 2018-01-23
- Earliest claimed: 2013-05-08
- Assignees: Novozymes A/S
- Applicants: Novozymes A/S
- Inventors: Mikkel Klausen; Klaus Skaalum Lassen
- Web page: https://patentgrep.com/patent/US9872892B2

## CPC

- A61K 38/443: {acting on CH-OH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)}
- A23K 10/14: Pretreatment of feeding-stuffs with enzymes
- A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10)
- A23K 20/142: Amino acids; Derivatives thereof
- A23K 20/174: Vitamins
- A23K 20/189: Enzymes
- A23K 20/195: Antibiotics
- A23K 20/20: Inorganic substances, e.g. oligoelements
- A23K 40/20: by moulding, e.g. making cakes or briquettes
- A23K 40/25: by extrusion
- A23K 50/75: for poultry
- A61K 38/44: Oxidoreductases (1)
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61P 1/12: Antidiarrhoeals
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

The present invention relates to methods of controlling the growth of microorganisms in animals and animal feed. In particular, the present invention relates to a method of controlling the growth of microorganisms in animals with an antimicrobial composition, the treatment of animal feed with an antimicrobial additive and antimicrobial compositions.

## Claims

### Claim 1

A method of controlling the growth of microorganisms in an animal comprising feeding the animal with an antimicrobial composition comprising a lactose oxidase and a vanadium haloperoxidase.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the vanadium haloperoxidase and the lactose oxidase are gastric stable.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the concentration of lactose oxidase is in the range of 0.01-200 ppm enzyme protein per kg feed.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the concentration of vanadium haloperoxidase is in the range of 0.01-200 ppm enzyme protein per kg feed.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the antimicrobial composition is a component of animal feed.

### Claim 6 (depends on 1)

The method according to claim 1, wherein controlling the growth of microorganism occurs in the gut of the animal.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the microorganism is a type of bacterium.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the bacterium is selected from the group consisting of Campylobacter, Clostridium, Escherichia, Listeria, and Salmonella.

### Claim 9 (depends on 1)

The method according to claim 1, wherein the composition further comprises one or more additional compounds selected from the group consisting of forage, concentrates, vitamins, minerals, amino acids, enzymes and other feed ingredients.

### Claim 10 (depends on 1)

The method according to claim 1, wherein the composition does not contain lactoferrin.

### Claim 11 (depends on 8)

The method according to claim 8, wherein said antimicrobial composition kills at least 90% of the number of bacterium.

### Claim 12 (depends on 8)

The method according to claim 8, wherein the bacterium is E. Coli.