# US 9,872,879 B2 · Process for the preparation of plant extracts for treating skin disorders and enhancing healing of wounds

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- Number: US 9,872,879 B2
- Document ID: US9872879B2
- Status: Granted
- Kind: B2
- Application number: 13/872,723
- Filed: 2013-04-29
- Published: 2013-11-21
- Granted: 2018-01-23
- Earliest claimed: 2007-08-29
- Assignees: Development Center for Biotechnology
- Applicants: Development Center for Biotechnology
- Inventors: Rey-Yuh Wu; Yuh-Shan Chung; Yu-Yuan Wu; Ma-Li Siu; Chin-Wen Hsiao
- Web page: https://patentgrep.com/patent/US9872879B2

## CPC

- A61K 36/53: Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K 36/23: Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention provides a process for the preparation of Plectranthus amboinicus extracts using a stirring separation method. The present invention also relates to a pharmaceutical composition comprising the Plectranthus amboinicus crude extract and/or extract for treating skin disorders, including enhancing the healing of wounds, especially in diabetic patients.

## Claims

### Claim 1

A process for preparing a Plectranthus amboinicus extract comprising the steps of:
a) contacting dried Plectranthus amboinicus leaves with 95% ethanol to obtain a Plectranthus amboinicus crude extract;
b) concentrating the Plectranthus amboinicus crude extract;
c) treating the concentrated Plectranthus amboinicus crude extract with a stirring separation method to obtain Plectranthus amboinicus extract, wherein the Plectranthus amboinicus extract is obtained without using column chromatography, and wherein the stirring separation method comprises the steps of:
(i) diluting the concentrated Plectranthus amboinicus crude extract with a high-polarity solvent to obtain a diluted extract, and stirring and dipping the diluted extract with a non-polar copolymer styrene-divynilbenzene adsorbent resin;
(ii) extracting the resin of (i) with distilled water and collecting a first extract;
(iii) further extracting the resin of (ii) with a sub-high-polarity solvent and collecting a second extract;
(iv) further extracting the resin of (iii) with a medium-polarity solvent and collecting a third extract; and
(v) further extracting the resin of (iv) with a low-polarity solvent and collecting a fourth extract; and
d) obtaining the Plectranthus amboinicus extract, wherein the Plectranthus amboinicus extract contains one or more extracts selected from the first, second, third and fourth extracts.