# US 9,872,878 B2 · Atractylodes lancea extract feed additive and preparation method thereof

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- Number: US 9,872,878 B2
- Document ID: US9872878B2
- Status: Granted
- Kind: B2
- Application number: 14/687,906
- Filed: 2015-04-15
- Published: 2015-10-22
- Granted: 2018-01-23
- Earliest claimed: 2014-04-16
- Assignees: Shanghai Zhao Xiang Biological Technology Co., LTD
- Applicants: Shanghai Zhao Xiang Biological Technology Co., LTD
- Inventors: Jiaming Chen; Shunjie Huang; Xuefeng Chen; Zili Chen; Ziqiang Chen; Ting Shu
- Web page: https://patentgrep.com/patent/US9872878B2

## CPC

- A61K 36/284: Atractylodes
- A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10)
- A23K 20/10: Organic substances
- A23K 20/163: Sugars; Polysaccharides
- A23K 20/28: Silicates, e.g. perlites, zeolites or bentonites
- A23K 40/00: Shaping or working-up of animal feeding-stuffs
- A23K 50/30: for swines
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

An Atractylodes Lancea extract feed additive and a preparation method thereof are provided. The Atractylodes Lancea extract feed additive takes an Atractylodes Lancea extract and a carrier as raw materials. The Atractylodes Lancea extract is prepared by the ultrasonic auxiliary water extraction. The preparation method of the present invention has less raw material waste, high activity component content in the extract and acquisition rate of the extractum, which facilitates improving the effect efficiency and reducing production cost. The Atractylodes Lancea extract feed additive as a natural plant feed additive is capable of reducing malaria incidence, restoring consciousness and relieving uneasiness, and diuretically swelling.

## Claims

### Claim 1

An Atractylodes Lancea extract feed additive, comprising an Atractylodes Lancea extract and a carrier, wherein the Atractylodes Lancea extract is prepared by a method comprising steps of:
(1) pretreating and smashing Atractylodes Lancea, immersing the Atractylodes Lancea for 5-6 h after adding ethanol solution, and then ultrasonically extracting at room temperature for 35-50 min through an ultrasonic extractor, and then filtering, and obtaining a filtrate and a first solid residue; and then vacuum evaporating for removing ethanol in the filtrate, and finally obtaining a first extracting solution;
(2) adding eight times an amount of water into the first solid residue, boiling for 4-6 h at a steam pressure of 0.4-0.7 MPa, filtering, and obtaining a second extracting solution and a second solid residue;
(3) adding five times an amount of water into the second solid residue, boiling for 1-3 h at a steam pressure of 1.5-1.6 MPa, filtering, and obtaining a third extracting solution and a third solid residue; and
(4) mixing the first extracting solution, the second extracting solution and the third extracting solution, and then concentrating and obtaining a first extractum; spraying and drying, and finally obtaining the Atractylodes Lancea extract.

### Claim 2 (depends on 1)

The Atractylodes Lancea extract feed additive, as recited in claim 1, wherein a weight ratio of the Atractylodes Lancea extract and the carrier is 1:3, and the carrier is a mixture of attapulgite and maltodextrin with a weight ratio of 1:(1-2).

### Claim 3

A method of preparing an Atractylodes Lancea extract feed additive, comprising steps of:
(1) pretreating and smashing Atractylodes Lancea, immersing the Atractylodes Lancea for 5-6 h after adding ethanol solution, and then ultrasonically extracting at room temperature for 35-50 min through an ultrasonic extractor, and then filtering, and obtaining a filtrate and a first solid residue; and then vacuum evaporating for removing ethanol in the filtrate, and finally obtaining a first extracting solution;
(2) adding eight times an amount of water into the first solid residue, boiling for 4-6 h at a steam pressure of 0.4-0.7 MPa, filtering to obtain a second extracting solution and a second solid residue;
(3) adding five times an amount of water into the second solid residue, boiling for 1-3 h at a steam pressure of 1.5-1.6 MPa, filtering to obtain a third extracting solution and a third solid residue;
(4) mixing the first extracting solution, the second extracting solution and the third extracting solution, concentrating for obtaining a first extractum; spraying and drying to obtain an Atractylodes Lancea extract dry powder; and
(5) evenly mixing the Atractylodes Lancea extract dry powder with a carrier to obtain the Atractylodes Lancea extract feed additive.

### Claim 4 (depends on 3)

The method, as recited in claim 3, wherein the step of pretreating and smashing Atractylodes Lancea comprises naturally drying and removing stems and leafs of the Atractylodes Lancea, drying for 6 h at 60° C., smashing, and sieving through a sieve with an aperture of 1.2-1.5 mm.

### Claim 5 (depends on 3)

The method, as recited in claim 3, wherein an amount of the added ethanol solution is 20-25 ml ethanol solution per gram Atractylodes Lancea, wherein a concentration of ethanol in the ethanol solution is 80 wt %-85 wt %.

### Claim 6 (depends on 4)

The method, as recited in claim 4, wherein an amount of the added ethanol solution is 20-25 ml ethanol solution per gram Atractylodes Lancea, wherein a concentration of ethanol in the ethanol solution is 80 wt %-85 wt %.

### Claim 7 (depends on 3)

The method, as recited in claim 3, wherein while ultrasonically extracting, an ultrasonic frequency is 110-120 KHz.

### Claim 8 (depends on 6)

The method, as recited in claim 6, wherein while ultrasonically extracting, an ultrasonic frequency is 110-120 KHz.

### Claim 9 (depends on 4)

The method, as recited in claim 4, wherein while ultrasonically extracting, moisture is distilled simultaneously for collecting a distillate.

### Claim 10 (depends on 8)

The method, as recited in claim 8, wherein while ultrasonically extracting, moisture is distilled simultaneously for collecting a distillate.

### Claim 11 (depends on 4)

The method, as recited in claim 4, wherein in the step (2), acetic acid is added to adjust a PH value of solution to 6.5, and in the step (3), saturated NaOH is added to adjust a PH value of solution to 8.0.

### Claim 12 (depends on 10)

The method, as recited in claim 10, wherein in the step (2), acetic acid is added to adjust a PH value of solution to 6.5, and in the step (3), saturated NaOH is added to adjust a PH value of solution to 8.0.

### Claim 13 (depends on 4)

The method, as recited in claim 4, wherein before spraying and drying, the step (4) further comprising steps of: eluting the first extractum with silica gel column chromatography gradient which takes petroleum ether/ethyl acetate as an eluent and eluting with petroleum ether/ethyl acetate at a ratio of 5:1, 3:1 and 2:1, and an eluting time of 15 min, combining eluting solutions, concentrating for obtaining a second extractum, spraying and drying the second extractum to obtain the Atractylodes Lancea extract dry powder.

### Claim 14 (depends on 12)

The method, as recited in claim 12, wherein before spraying and drying, the step (4) further comprising steps of: eluting the first extractum with silica gel column chromatography gradient which takes petroleum ether/ethyl acetate as an eluent and eluting with petroleum ether/ethyl acetate at a ratio of 5:1, 3:1 and 2:1, and an eluting time of 15 min, combining eluting solutions, concentrating for obtaining a second extractum, spraying and drying the second extractum to obtain the Atractylodes Lancea extract dry powder.

### Claim 15 (depends on 3)

The method, as recited in claim 3, wherein a weight ratio of the Atractylodes Lancea extract dry power and the carrier is (1-2):(3-5).

### Claim 16 (depends on 13)

The method, as recited in claim 13, wherein a weight ratio of the Atractylodes Lancea extract dry power and the carrier is (1-2):(3-5).

### Claim 17 (depends on 14)

The method, as recited in claim 14, wherein a weight ratio of the Atractylodes Lancea extract dry power and the carrier is (1-2):(3-5).

### Claim 18 (depends on 4)

The method, as recited in claim 4, wherein the carrier is a mixture of attapulgite and maltodextrin with a weight ratio of 1:(1-2).

### Claim 19 (depends on 17)

The method, as recited in claim 17, wherein the carrier is a mixture of attapulgite and maltodextrin with a weight ratio of 1:(1-2).