# US 9,872,477 B1 · Maize hybrid X80H133

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- Number: US 9,872,477 B1
- Document ID: US9872477B1
- Status: Granted
- Kind: B1
- Application number: 15/447,595
- Filed: 2017-03-02
- Granted: 2018-01-23
- Earliest claimed: 2017-03-02
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International, Inc.
- Inventors: Louis Brian Chapko; Steven Paul King; Lori Karyn Scott
- Web page: https://patentgrep.com/patent/US9872477B1

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8246: {Non-starch polysaccharides, e.g. cellulose, fructans, levans}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8286: {for insect resistance}
- C12N 15/8289: {Male sterility}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A novel maize variety designated X80H133 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X80H133 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X80H133 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X80H133, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X80H133. This invention further relates to methods for producing maize varieties derived from maize variety X80H133.

## Claims

### Claim 1

A seed of hybrid maize variety X80H133, representative seed produced by crossing a first plant of variety PH26B5 with a second plant of variety PH1DAM, wherein representative seed of the varieties PH26B5 and PH1DAM have been deposited under ATCC Accession Numbers PTA-124329 and PTA-120428, respectively.

### Claim 2 (depends on 1)

A plant of hybrid maize variety X80H133 grown from the seed of claim 1.

### Claim 3 (depends on 2)

A plant part or cell of the plant of claim 2, wherein the plant part comprises at least one cell of hybrid maize variety X80H133.

### Claim 4 (depends on 1)

A method of producing the seed of claim 1, the method comprising crossing a plant of variety PH26B5 with a plant of variety PH1DAM, wherein representative seed of varieties PH26B5 and PH1DAM have been deposited under ATCC Accession numbers PTA-124329 and PTA-120428, respectively.

### Claim 5

A maize seed produced by crossing a plant of variety PH26B5, or a selfed progeny thereof, having a transgene introduced by backcrossing or transformation, with a plant of variety PH1DAM, (ii) a plant of variety PH1DAM, or a selfed progeny thereof, having a transgene introduced by backcrossing or transformation, with a plant of variety PH26B5, or (iii) a plant of variety PH26B5, or a selfed progeny thereof, having a transgene introduced by backcrossing or transformation, with a plant of variety PH1DAM, or a selfed progeny thereof, having a transgene introduced by backcrossing or transformation, wherein the backcrossing is sufficient to produce a first or second plant further comprising the transgene and otherwise all of the morphological and physiological characteristics of maize variety PH1DAM or maize variety PH26B5, and wherein representative seed of the varieties PH26B5 and PH1DAM have been deposited under ATCC Accession Numbers PTA-124329 and PTA-120428, respectively and wherein the transgene is inherited by the maize seed.

### Claim 6 (depends on 5)

The seed of claim 5, wherein the transgene confers a property selected from the group consisting of male sterility, herbicide tolerance, insect resistance, disease resistance, waxy starch, modified fatty acid metabolism, modified phytic acid metabolism, modified carbohydrate metabolism and modified protein metabolism.

### Claim 7 (depends on 5)

The seed of claim 5, further comprising a seed treatment on the surface of the seed.

### Claim 8 (depends on 5)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the seed of claim 5.

### Claim 9 (depends on 5)

A plant grown from the seed of claim 5.

### Claim 10 (depends on 9)

A method for producing a second maize plant, the method comprising applying plant breeding techniques to the plant of claim 9 to produce the second maize plant.

### Claim 11 (depends on 9)

A method for producing a second maize plant, the method comprising doubling haploid seed generated from a cross of the maize plant of claim 9 with a different maize plant.

### Claim 12

An maize seed produced by crossing (i) a plant of variety PH26B5, or a selfed progeny thereof, having a locus conversion introduced by backcrossing or transformation, with a second plant of variety PH1DAM, (ii) a plant of variety PH1DAM, or a selfed progeny thereof, having a locus conversion introduced by backcrossing or transformation, with a plant of variety PH26B5, or (iii) a plant of variety PH26B5, or a selfed progeny thereof, having a locus conversion introduced by backcrossing or transformation, with a plant of variety PH1DAM, or a selfed progeny thereof, having a locus conversion introduced by backcrossing or transformation, wherein the backcrossing is sufficient to produce a first or second plant further comprising the locus conversion and otherwise all of the morphological and physiological characteristics of maize variety PH1DAM or maize variety PH26B5; representative seed of the varieties PH26B5 and PH1DAM having been deposited under ATCC Accession Numbers PTA-124329 and PTA-120428, respectively; wherein the seed produces a plant having otherwise essentially all the physiological and morphological characteristics of maize variety X80H133 when grown under the same environmental conditions, and wherein the locus conversion is inherited by the maize seed.

### Claim 13 (depends on 12)

The seed of claim 12, further comprising a seed treatment on the surface of the seed.

### Claim 14 (depends on 12)

The seed of claim 12, wherein the locus conversion confers a property selected from the group consisting of male sterility, herbicide tolerance, insect resistance, disease resistance, waxy starch, modified fatty acid metabolism, modified phytic acid metabolism, modified carbohydrate metabolism and modified protein metabolism.

### Claim 15 (depends on 12)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the seed of claim 12.

### Claim 16 (depends on 12)

A plant, plant part, or plant cell produced by growing the seed of claim 12, the plant, plant part or plant cell comprising at least one F1 hybrid maize variety X80H133 cell.

### Claim 17 (depends on 16)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the plant, plant part, or plant cell of claim 16.

### Claim 18 (depends on 16)

A method of producing a commodity plant product comprising starch, syrup, silage, fat or protein, the method comprising producing the commodity plant product from the plant or plant part of claim 16.

### Claim 19 (depends on 16)

A method for producing a second maize plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 16 to produce the second maize plant.

### Claim 20 (depends on 16)

A method for producing a second maize plant, the method comprising doubling haploid seed generated from a cross of the maize plant or plant part of claim 16 with a different maize plant.