Compositions and methods for mass spectometry
OwnerSarah Trimpininventor
Life of this patent
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Abstract
The invention provides ionizing matrix compounds. These compounds are useful for mass spectrometry and ion mobility spectrometry as ionizing matrices facilitating transfer of diverse classes of analyte compounds from solid or solution states to gas-phase ions.
Claims20
A method of ionizing an analyte molecule, the method comprising:
- providing an ionizing matrix comprising one or more of the compounds 1-(chloromethyl)-4-nitrobenzene, (phenyl sulfonyl)acetonitrile, 3-(methylsulfonyl)benzonitrile, 1-(methylsulfonyl)piperidine-3-carboxylic acid, 2-(methylsulfonyl)acetonitrile, 4-[(2-hydroxyethyl)amino]tetrahydrothiophene-3-ol-1,1-dioxide, 2-(methylsulfonyl)phenol, 4-[(2-hydroxyethyl)namino]etrahydrothiophene-3-ol 1,1 dioxide, tetrahydrothiophene-3-ol-1,1-dioxide, 3-bromotetrahydrothiophene 1,1-dioxide, (3S,4S)-3-hydroxy-4-(methylamino)tetrahydrothiophene 1,1-dioxide, tetrahydrothiophene 1,1-dioxide, 2-pyridinecarbonitrile, 9-isopropyl-9H-carbazole, 9-vinyl-9H-carbazole, 3,6-dibromo-9H-carbazole, 2,5-diphenyl-1,3,4-oxadiazole, 2,5-dimethylbenzoyl chloride, 2,5-diphenyl-1,3,4-oxadiazole, 3,6-dibroino-9H-carbazole, 2,5-dimethoxybenzoyl chloride, 2,5-dimethylbenzoic acid, 1-(5-bromo-2-hydroxyphenyl)ethan-1 -one, 2-bromo-1-(2-hydroxyphenyl)ethan-1-one, 3-(trifluoromethyl)benzonitrile, 3-methoxybenzonitrile, 4-methyl-1,2-dicyanobenzene, 4-amino-1,2-dicyanobenzene, 1,2-dicyanobenzene, 3-nitrobenzoic acid, 3-nitro-1,2-dicyanobenzene, 3-nitroaniline, 1-(2-nitrophenyl)ethan-1-one, 1-methyl-4-(methylsulfonyl)-2-nitrobenzene, nitrocyclohexane, 5-bromo-5-nitro-1,3-dioxane, 2-bromo-2-nitropropane, methyl-4-methyl-4-nitropentanoate, 2-methyl-2-nitropropan-1,3-diol, 5-nitro-1H-indole, 4-hydroxy-3-nitro-2H-chromen-2-one, 1,3,5-trifluoro-2-nitrobenzene, 1,3-dimethyl-2-nitrobenzene, 4-nitro-1,2-bis(trifluoromethyl)benzene, 5-methoxy-2-nitro aniline, 4-chloro-1-nitro -2-(trifluoromethyl)benzene, 2,4-dinitrophenol, 1-methoxy-2-nitrobenzene, 1-nitro-2-(pentyloxy)benzene, 5-bromo-3-nitropicolinonitrile, 1-nitro -3-vinylbenzene, 4-methyl-3-nitroaniline, 1-(2-hydroxyl-5-methyl-3-nitrophenyl)ethan-1-one, 2-bromo-1-(3-nitrophenyl)ethan-1-one, 3-nitrobenzoyl chloride, (3-nitrophenyl)methanol, 1-(4-hydroxy1-3-nitrophenyl)ethan-1-one, 1-(2-hydroxyl-5-nitrophenyl)ethan-1-one, 3,5-dinitrobenzoyl chloride, 1,3-dinitrobenzene, and 4-chloro-3-nitrobenzonitrile, or a derivative of these compounds;
- contacting the ionizing matrix with an analyte to form a sample comprising an analyte and an ionizing matrix;
- and
- subliming or evaporating the ionizing matrix in the sample to produce gas-phase positive or negative ions of the analyte.
A method for producing gas-phase analyte ions for analysis by mass spectrometry or ion mobility spectrometry, the method comprising:
- mixing an ionizing matrix comprising one or more of the compounds 1-(chloromethyl)-4-nitrobenzene, (phenylsulfonyl)acetonitrile, 3-(methylsulfonyl)benzonitrile, 1-(methylsulfonyl)piperidine-3-carboxylic acid, 2-(methylsulfonyl)acetonitrile, 4-[(2-hydroxyethyl)amino]tetrahydrothiophene-3-ol-1,1-dioxide, 2-(methylsulfonyl)phenol, 4-[-(2-hydroxyethyl)amino]tetrahydrothiophene-3-ol 1,1 dioxide, tetrahydrothiophene-3-of-1,1-dioxide, 3-bromotetrahydrothiophene 1,1-dioxide, (3S,4S)-3-hydroxy-4-(methylamino)tetrahydrothiophene 1,1-dioxide, tetrahydrothiophene 1,1-dioxide, 2-pyridinecarbonitrile, 9-isopropyl-9H-carbazole, 9-vinyl-9H-carbazole, 3,6-dibromo-9H-carbazole, 2,5-diphenyl-1,3,4-oxadiazole, 2,5-dimethylbenzoyl chloride, 2,5-diphenyl-1,3,4-oxadiazole, 3,6-dibromo-9H-carbazole, 2,5-dimethoxybenzoyl chloride, 2,5-dimethylbenzoic acid, 1-(5-bromo-2-hydroxyphenyl)ethan-1-one, 2-bromo-1-(2-hydroxyphenyl)ethan-1-one, 3-(trifluoromethyl)benzonitrile, 3-methoxybenzonitrile, 4-methyl-1,2-dicyanobenzene, 4-amino-1,2-dicyanobenzene, 1,2-dicyanobenzene, 3-nitrobenzoic acid, 3-nitro-1,2-dicyanobenzene, 3-nitroaniline, 1-(2-nitrophenyl)ethan-1-one, 1-methyl-4-(methylsulfonyl)-2-nitrobenzene, nitrocyclohexane, 5-bromo-5-nitro-1,3-dioxane, 2-bromo-2-nitropropane, methyl-4-methyl-4-nitropentanoate, 2-methyl-2-nitropropan-1,3-diol, 5-nitro-1H-indole, 4-hydroxy-3-nitro-2H-chromen-2-one, 1,3,5-trifluoro-2-nitrobenzene, 1,3-dimethyl-2-nitrobenzene, 4-nitro-1,2-bis(trifluoromethyl)benzene, 5-methoxy-2-nitroaniline, 4-chloro-1-nitro-2-(trifluoromethyl)benzene, 2,4-dinitrophenol, 1-methoxy-2-nitrobenzene, 1-nitro-2-(pentyloxy)benzene, 5-bromo-3-nitropicolinonitrile, 1-nitro-3-vinylbenzene, 4-methyl-3-nitroaniline, 1-2-hydroxyl-5-methyl-3-nitrophenyl)ethan-1-one, 2-bromo-1-(3-nitrophenyl)ethan-1-one, 3-nitrobenzoyl chloride, (3-nitrophenyl)methanol, 1-(4-hydroxyl-3-nitrophenyl)ethan-1-one, 1-(2-hydroxyl-5-nitrophenypl)ethan-1-one, 3,5-dinitrobenzoyl chloride, 1,3-dinitrobenzene, and 4-chloro-3-nitrobenzonitrile, and an analyte to produce a matrix:analyte sample;
- and
- exposing the matrix:analyte sample to sub-atmospheric pressure to initiate charge separation and production of analyte ions from the matrix:analyte sample.
A composition comprising an ionizing matrix compound from the compounds 1-(chloromethyl)-4-nitrobenzene, (phenyl sulfonyl)acetonitrile, 3-(methylsulfonyl)benzonitrile, 1-(methylsulfonyl)piperidine-3-carboxylic acid, 2-(methylsulfonyl)acetonitrile, 4-[(2-hydroxyethypamino]tetrahydrothiophene-3-ol-1,1-dioxide, 2-(methylsulfonyl)phenol, 4-[(2-hydroxyethyl)amino]tetrahydrothiophene-3-ol 1,1 dioxide, tetrahydrothiophene-3-ol -1,1-dioxide, 3-bromotetrahydrothiophene 1,1-dioxide, (3S,4S)-3-hydroxy-4-(methylamino)tetrahydrothiophene 1,1-dioxide, tetrahydrothiophene 1,1-dioxide, 2-pyridinecarbonitrile, 9-isopropyl-9H-carbazole, 9-vinyl-9H-carbazole, 3,6-dibromo-9H-carbazole, 2,5-diphenyl-1,3,4-oxadiazole, 2,5-dimethylbenzoyl chloride, 2,5-diphenyl-1,3,4-oxadiazole, 3,6-dibromo-9H-carbazole, 2,5-dimethoxybenzoyl chloride, 2,5-dimethylbenzoic acid, 1-(5-bromo-2-hydroxyphenyl)ethan-1-one, 2-bromo-1-(2-hydroxyphenynethan-1-one, 3-(trifluoromethyl)benzonitrile, 3-methoxybenzonitrile, 4-methyl-1,2-dicyanobenzene, 4-amino-1,2-dicyanobenzene, 1,2-dicyanobenzene, 3-nitrobenzoic acid, 3-nitro-1,2-dicyanobenzene, 3-nitroaniline, 1-(2-nitrophenyl)ethan-1-one, 1-methyl-4-(methylsulfonyl)-2-nitrobenzene, nitrocyclohexane, 5-bromo-5-nitro-1,3-dioxane, 2-bromo-2-nitropropane, methyl-4-methyl-4-nitropentanoate, 2-methyl-2-nitropropan-1,3-diol, 5-nitro-1H-indole, 4-hydroxy-3-nitro-2H-chromen-2-one, 1,3,5-trifluoro-2-nitrobenzene, 1,3-dimethyl-2-nitrobenzene, 4-nitro-1,2-bis(trifluoromethyl)benzene, 5-methoxy-2-nitroaniline, 4-chloro-1-nitro-2-(trifluoromethyl)benzene, 2,4-dinitrophenol, 1-methoxy-2-nitrobenzene, 1-nitro-2-(pentyloxy)benzene, 5-bromo-3-nitropicolinonitrile, 1-nitro-3-vinylbenzene, 4-methyl-3-nitroaniline, 1-(2-hydroxy1-5-methyl-3-nitrophenyl)ethan-1-one, 2-bromo-1-(3-nitrophenyl)ethan-1-one, 3-nitrobenzoyl chloride, (3-nitrophenyl)methanol, 1-(4-hydroxyl-3-nitrophenyl)ethan-1-one, 1-(2-hydroxyl-5-nitrophenyl)ethan-1-one, 3,5-dinitrobenzoyl chloride, 1,3-dinitrobenzene, and 4-chloro-3-nitrobenzonitrile, and an analyte.
Dependencies are inferred from the claim text.