Analyzing bacteria without culturing
AssigneeDNAE Group Holdings Limited
InventorsSergey A. Dryga, Lisa-Jo Ann Clarizia, Eddie W. Adams, Victor C. Esch
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Abstract
The invention generally relates to conducting an assay on a sample that isolates a pathogen from the sample and allows for analysis of the pathogen with minimal (i.e., at most 24 hrs of culturing) or no culturing of the pathogen. In certain embodiments, the invention provides methods for identifying a pathogen from a sample that involve obtaining a sample including a pathogen, conducting an assay that isolates the pathogen from the sample, culturing the isolated pathogen for at most about 24 hrs, and analyzing the pathogen.
Claims17
A method for analyzing a bacterium from a sample, the method comprising:
- providing a vessel that contains a body fluid sample comprising a bacterium;
- introducing to the sample in the vessel, magnetic particles comprising a bacterium-specific binding moiety and a buffer that promotes binding of the particles to the bacterium in order to create a mixture containing bacterium/magnetic particle complexes;
- flowing the mixture from the vessel into a fluidic device through a channel in the fluidic device and toward a magnet;
- applying a magnetic field to isolate the bacterium/magnetic particle complexes from the sample;
- and
- analyzing the bacterium.
A method for detecting a bacterium from a sample, the method comprising:
- providing a vessel that contains a body fluid sample comprising a bacterium;
- introducing to the sample in the vessel, magnetic particles comprising a bacterium-specific binding moiety and a buffer that promotes binding of the particles to the bacterium in order to create a mixture;
- incubating the mixture in the vessel to allow the particles to bind to the bacterium in the sample and form bacterium/magnetic particle complexes;
- flowing the mixture from the vessel into a fluidic device through a channel and toward a magnet;
- applying a magnetic field to isolate bacterium/magnetic particle complexes from the sample;
- and
- detecting the bacterium.
Dependencies are inferred from the claim text.