Hydrogel encapsulated cell patterning and transferring method and cell-based biosensor using the same

A method of patterning and transferring of hydrogel-encapsulated cells, comprising:

• preparing a first substrate having a multiple hydrogel-encapsulated cell patterns, each of which comprising first cells and an alginate hydrogel, wherein the first substrate is formed by,
• forming a first mold having a photosensitive resin on the first substrate by soft lithography,
• preparing a second mold having a shape corresponding the first mold, followed by pouring a polymer thereinto and heat treating the result so as to form a polymer mold,
• filling the first cells simultaneously mixed with an alginate hydrogel solution into compartmentalized wells of the polymer mold,
• tilting the polymer mold to remove the excess of the solution, and
• gelating the multiple hydrogel-encapsulated cell patterns to provide the hydrogel-encapsulated cells by immersing the mixed first cells and the alginate hydrogel solution in a calcium solution,
• wherein the hydrogel-encapsulated cells form hydrogel microparticles,
• wherein each hydrogel-encapsulated cell pattern has a shape corresponding to each compartmentalized well of the polymer mold,
• wherein an aspect ratio (AR=diameter/height) of each hydrogel microparticle is greater than 1.25;
• preparing a second planar substrate comprising agarose hydrogel and any one of a second cell and a physiological active substance;
• transferring and placing the first substrate on the second planar substrate;
• and
• peeling off the polymer mold so that the hydrogel-encapsulated cells are left on the second planar substrate, wherein the hydrogel-encapsulated cells are compartmentalized on the second planar substrate.