Method to assess human allograft status from microrna expression levels
AssigneeCornell University
InventorManikkam Suthanthiran
Life of this patent
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Abstract
The invention relates to, among other things, a method for assessing risk of organ rejection in a patient having a transplanted organ. The method includes measuring an amount of expression of a small non-coding marker RNA in a biological sample from the patient. The method further includes comparing the measured amount of expression of the small non-coding marker RNA in the patient to a reference amount of expression of the small non-coding marker RNA. In another aspect, the invention relates to kits for assessing risk of organ rejection in a patient having a transplanted organ.
Claims14
A method comprising:
- (a) measuring expression of one or a combination of small non-coding marker RNAs in a sample comprising kidney, blood, and/or urine from a patient by quantitative polymerase chain reaction to generate a measured amount of expression, wherein said one or a combination of small non-coding marker RNA(s) is selected from the group of SEQ. ID NOs:
- 1-9 and 50;
- b) quantifying a difference between the measured amount of expression in step (a) and a reference amount of expression of said one or a combination of small non-coding marker RNAs in a person having a non-rejected organ or a second biological sample from the patient;
- and
- (c) administering an anti-rejection treatment to the patient to reduce a risk of kidney transplant rejection when an increase of expression of at least two-fold is detected of said small non-coding marker RNA(s) in the sample compared to said reference amount.
The method according to 1, wherein said reference amount is obtained by measuring an amount of expression of said small non-coding marker RNA in a second biological sample from the patient.
Dependencies are inferred from the claim text.