Loading patent…
AssigneeJiangnan University
InventorsLong Liu, Jian Chen, Guocheng Du, Jianghua Li, Yang Gu, Yang Song, Jieying Deng, Yawen Zhao
Estimating the term…
The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineered B. subtilis Deletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineered B. subtilis for GlcNAc production, which can be further applied to industrial production of GlcNAc.
A method for improving GlcNAc production of recombinant Bacillus Subtilis comprising deletion of phosphoenolpyruvate carboxykinase encoding gene pckA, deletion of pyruvate kinase encoding gene pyk, as well as overexpression of pyruvate carboxylase encoding gene pycA in the recombinant Bacillus Subtilis.
Dependencies are inferred from the claim text.