Genetically engineered yeast
AssigneeTechnical University of Denmark
InventorsIrina Borodina, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Fredrik Öberg
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Abstract
A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalyzing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.
Claims8
A genetically modified yeast cell comprising an enhanced-fermentation pathway for producing 3-hydroxypropionic acid (3HP), wherein the fermentation pathway includes an enzyme having at least 85% identity with SEQ ID NO:
- 1, and catalysing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde, and wherein said enzyme is the expression product of an exogenous gene that is expressed by the genetically modified yeast cell.
Dependencies are inferred from the claim text.