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AssigneesNorth Carolina State University, Medical Research Council
InventorsDuy P. Nguyen, Heinz Neumann, Alexander Deiters, Jason Chin, Hrvoje Lusic
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The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.
A method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid comprising an alkyne group or an azide group, wherein said aliphatic amino acid does not comprise an aromatic moiety, said method comprising providing a host cell;
A method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid comprising an alkyne group or an azide group, wherein the aliphatic amino acid does not comprise an aromatic moiety, said method comprising providing a host cell;
Dependencies are inferred from the claim text.