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AssigneeIowa State University Research Foundation, Inc.
InventorsBasil J. Nikolau, Marna Yandeau-Nelson, Fuyuan Jing
Estimating the term…
A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.
An isolated or purified nucleic acid molecule comprising a nucleotide sequence encoding a mutant plant acyl-acyl carrier protein thioesterase (acyl-ACP TE), the amino acid sequence of which is at least 85% identical to the amino acid sequence of SEQ ID NO:3 and which comprises a substrate specificity-altering mutation of at least one amino acid corresponding to an amino acid selected from the group consisting of amino acid 133, amino acid 139, amino acid 142, and amino acid 143 of SEQ ID NO:3, alone or in further combination with a mutation of at least one amino acid in the region corresponding to amino acids 73-85 of SEQ ID NO:3,
An isolated or purified nucleic acid molecule comprising a nucleotide sequence encoding a mutant plant acyl-ACP TE, the amino acid sequence of which is at least 85% identical to the amino acid sequence of SEQ ID NO:3 and which comprises:
Dependencies are inferred from the claim text.