Bioassay for cell conditioned media
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsRobert M. Hnasko, Larry H. Stanker, Jeffery A. McGarvey, Alice V. Lin
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Abstract
Herein is described a novel hybridoma cell line (RMH359) and its use as a cellular bioassay to determine the efficacy of cell conditioned media (CCM) as a supplement used to support cell survival and promote growth in culture. This bioassay is the first to provide a measure of CCM bioactivity in support of hybridoma cells used in the generation and production of monoclonal antibodies.
Claims2
A composition comprising a conditioned media dependent hybridoma cell line deposited under accession number ATCC PTA-122116 and a cell conditioned media.
A bioassay method for testing a macrophage conditioned media to determine whether said macrophage conditioned media promotes growth and sustains cell viability of cells of hybridoma cell line deposited under accession number ATCC PTA-122116, the method comprising:
- (1) producing a macrophage conditioned media;
- (2) culturing cells of hybridoma cell line deposited under accession number ATCC PTA-122116;
- (3) seeding the cultured cells of the hybridoma cell line of (2) with the macrophage conditioned media of (1);
- and
- (4) measuring cell growth and survival of the cultured cells seeded in (3) to determine cell viability of said cultured cells, thereby testing the macrophage conditioned media.
Dependencies are inferred from the claim text.