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US 9,868,939 B2Granted
Generating vasculogenic cell populations
AssigneeWisconsin Alumni Research Foundation
InventorsIgor Slukvin, Akhilesh Kumar
Life of this patent
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Abstract
The present invention relates generally to methods and compositions useful for therapeutic vascular tissue engineering. In particular, the present invention provides methods for generating substantially pure populations of vasculogenic cells from human mesenchymal progenitors, and methods and compositions for clinical applications in the field of regenerative medicine.
Claims10
A method for generating an isolated population of primate vasculogenic cells, the method comprising:
- culturing a primate mesenchymoangioblast (MAB)-derived colony comprising PDGFRβ+ (Platelet Derived Growth Factor Receptor β+)/EMCNhigh/CD105low/CD248−/CD73−/CD31− mesenchymal progenitors on a culture plate having a coating comprising fibronectin, gelatin or collagen, in a serum-free culture medium comprising basic fibroblast growth factor (FGF2) and Platelet-Derived Growth Factor BB (PDGF-BB) in an amount effective to promote differentiation of the mesenchymal progenitors to obtain vasculogenic immature pericytes having a RGS5+ (Regulator of G-protein Signaling 5+)/PDGFRβ+/ANG-1+ (angiopoietin-1+)/CD 146+/CD44+/CD 13+/NG2+ (Nerve/Glial antigen 2+)/CD73+ expression profile;
- and
- (b) isolating the vasculogenic immature pericytes.
Dependencies are inferred from the claim text.