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US 9,868,938 B2Granted
Methods for accelerated and enhanced cardiac differentiation of IPS cells by electrical stimulation
AssigneeUniversity of Cincinnati
InventorsYi-Gang Wang, Ha Won Kim, Ruilian Ma
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Abstract
Methods of generating cardiomyocytes from induced pluripotent stem cells (IPSCs) are provided. More specifically, the present disclosure relates to methods of generating cardiomyocytes from iPSCs using electrical stimulation. In some aspects, uses of such cells for therapeutics and in methods of treatment are provided.
Claims9
An in vitro method for the accelerated and enhanced generation of cardiomyocytes, the method comprising:
- applying electrical stimulation to an embryoid body (EB) of human induced pluripotent stem cells (iPSCs) for a time period to induce cardiomyocyte differentiation of the induced pluripotent stem cells to form cardiomyocytes without the use of exogenous growth factors, wherein the exogenous growth factors are selected from the group consisting of bone morphogenetic proteins, wingless/INT proteins, fibroblastic growth factors, vascular endothelial growth factor, activin A and ascorbic acid/vitamin C, wherein generation of cardiomyocytes from human iPSCs is accelerated and enhanced when compared to in vitro generation from human iPSCs without applying electrical stimulation.
An in vitro method for the accelerated and enhanced generation of cardiomyocytes, the method comprising:
- seeding human induced pluripotent stem cells on a substrate;
- cultivating the seeded human induced pluripotent stem cells without exogenous growth factors, wherein the exogenous growth factors selected from the group consisting of bone morphogenetic proteins, wingless/INT proteins, fibroblastic growth factors, vascular endothelial growth factor, activin A and ascorbic acid/vitamin C;
- and
- applying electrical stimulation to an embryoid body (EB) of the seeded human induced pluripotent stem cells for a time period to induce cardiomyocyte differentiation of the induced pluripotent stem cells to form cardiomyocytes, wherein generation of cardiomyocytes from human induced pluripotent stem cells is accelerated and enhanced when compared to generation from human induced pluripotent stem cells without applying electrical stimulation.
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