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US 9,868,936 B2Granted
Cell culture method
AssigneeIsopogen Pty Ltd
InventorMarian June Sturm
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Abstract
The present invention is directed towards methods for producing cell and tissue compositions suitable for therapeutic applications to a mammal in need of a therapeutic cell or tissue treatment. In particular, the invention is directed towards a method of culturing cells for use in therapy, which method comprises (i) culturing a sample of cells in a first cell culture medium and (ii) prior to harvesting cells for use in therapy culturing the cells in a second cell culture medium.
Claims6
A method of culturing human mesenchymal stromal cells for use in allogeneic therapy comprising the steps of:
- (i) providing a sample of said cells from a donor to a first culture medium;
- (ii) culturing said cells in the first cell culture medium comprising fetal calf serum, for at least 18 hours until said cells are at least 70% confluent;
- (iii) replacing said first cell culture medium with a second cell culture medium comprising about 2% v/v human serum albumin;
- (iv) further culturing the cells for between 24 hours and 36 hours in the second cell culture medium;
- and
- (v) harvesting said cells for use in said allogeneic therapy.
3 more elements
Dependencies are inferred from the claim text.