Cell culturing method, cell culturing apparatus and kit comprising a porous polyimide film

Abstract

The present invention relates to a cell culturing method and cell culturing apparatus and kit for use therewith. The method includes applying cells on a porous polyimide film and culturing. One embodiment includes a process for sowing cells on the surface of the film. Another embodiment includes a process for placing a cell suspension on the dried surface of the film and leaving the film undisturbed, moving the film to promote liquid efflux, or stimulating a portion of the surface to entangle the cell suspension into the film, and then retaining the cells in suspension inside the film while allowing moisture to flow out. Another embodiment includes a process for moistening one or both surfaces of the film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened film, retaining the cells in suspension inside the film, and allowing moisture to flow out.

Dependencies are inferred from the claim text.