Method for purifying virus-like particles (VLP)
AssigneeLife Science Inkubator Betriebs GmbH & Co. KG
InventorsVictoria Demina, Heiko Manninga
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Abstract
The invention relates to a method for purifying compositions containing virus-like particles (VLP), wherein a VLP-containing composition is filtered through a filter medium, in particular through a membrane, having a molecular weight cut off (MWCO) of more than 30 kDa, and the cell culture supernatant of VLP-expressing cells is used as a VLP-containing composition. The invention further relates to a VLP-containing composition which can be produced by this method.
Claims14
A method for purifying virus-like particles (VLPs) of human polyomavirus JC (JCV) comprising a structural protein VP1, the method comprising:
- filtering a VLP-containing composition through a filter medium having an exclusion limit of 30 kDa to 1500 kDa;
- dissociating the virus-like particles (VLPs) so as to obtain VP1 pentamers;
- purifying the VP1 pentamers by anion exchange chromatography so as to obtain purified VP1 pentamers;
- and
- reassociating the purified VP1 pentamers so as to obtain reassociated virus-like particles (VLPs),
- wherein,
- the VLP-containing composition is a supernatant of a culture of VLP-expressing cells (cell culture supernatant),
- a buffer exchange is achieved during the filtering, and
- before filtration, the cell culture supernatant is not chromatographed.
Dependencies are inferred from the claim text.