Back to results
US 9,868,761 B2Granted
Buffer system for protein purification
AssigneeGlaxoSmithKline LLC
InventorsKent E. Goklen, Eric J. Suda, Antonio Raul Ubiera
Life of this patent
Estimating the term…
Abstract
A method for purifying a protein using a simplified, sodium chloride-free buffer system that consists of two components (acid and base pairs) for appropriate solution pH control; and a third component for ionic strength control, where the third component is the sodium salt conjugate base.
Claims18
A method for purifying a protein from a contaminated solution thereof comprising the steps of:
- (1) passing the protein through a series of chromatography steps operated in a mode chosen from:
- bind-elute mode and flow-through mode, using a sodium chloride-free multi-component buffer system,
- wherein the chromatography steps comprise affinity chromatography and at least one additional chromatography step chosen from:
- anion exchange chromatography, cation exchange chromatography, and mixed-mode chromatography, and
- wherein the sodium chloride-free multi-component buffer system comprises:
- (a) an organic acid,
- (b) at least one component chosen from an alkaline metal and an ammonium salt of the conjugate base of the organic acid of (a), and
- (c) an organic base;
- and
- wherein the sodium chloride-free multi-component buffer system is used throughout the series of chromatography steps;
- and
- (2) recovering the purified protein.
9 more elements
Dependencies are inferred from the claim text.