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US 9,868,760 B2Granted
Protein purification
AssigneeGenentech, Inc.
InventorsJefferson C. Emery, Paul J. McDonald, Rhona O'Leary
Life of this patent
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Abstract
A method for purifying a polypeptide by ion exchange chromatography is described in which a gradient wash is used to resolve a polypeptide of interest from one or more contaminants.
Claims11
A method for purifying a polypeptide from a composition comprising the polypeptide and a contaminant, which method comprises the following steps performed sequentially:
- (a) loading the composition onto an ion exchange material;
- (b) washing the ion exchange material with wash buffer using a multi-slope gradient, wherein the multi-slope gradient comprises two or more segments of linear salt gradients with different slopes wherein the slope is greater in the first segment than in any additional segments, the increase in the salt concentration of the wash buffer is greater in the first segment of the multi-slope gradient wash than in subsequent segments, and each segment of the multi-slope wash ends when a predetermined polypeptide concentration is measured in the flowthrough, wherein each segment of the multi-slope gradient has a progressively shallower slope;
- and
- (c) eluting the polypeptide from the ion exchange material.
1 more element
Dependencies are inferred from the claim text.