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US 9,867,877 B2Granted
Methods for preparing squalene
AssigneeNovartis AG
InventorManinder Hora
Life of this patent
Estimating the term…
Abstract
An improved method for preparing squalene from a squalene-containing composition, said method comprising the steps of (a) a purification distillation carried out at a temperature T1 (b) a denaturing distillation carried out at a temperature T2; wherein steps (a) and (b) may be performed in either order; T1 and T2 are sufficient to cause squalene to boil; T2>T1; and T2>200° C.
Claims33
A method for the manufacture of an oil-in-water emulsion comprising:
- (i) preparing purified squalene from a composition comprising squalene from a fish source by a process comprising steps of:
- (a) a purification distillation carried out at a temperature less than 140° C.;
- and (b) a denaturing distillation carried out at a temperature greater than or equal to 200° C.;
- wherein the purification step and the denaturing distillation step may be performed in either order;
- the temperature of the purification step and the denaturing distillation step is sufficient to cause the squalene to boil;
- and the denaturing distillation denatures and/or removes potential contaminant proteins and viruses, thereby rendering the squalene safe for human use as compared with non-denatured squalene;
- and
- (ii) preparing an oil-in-water emulsion using the squalene prepared in step (i).
5 more elements
A method for the manufacture of an oil-in-water emulsion adjuvant comprising:
- (i) preparing purified squalene from a composition comprising squalene from a fish source by a process comprising steps of:
- (a) a purification distillation carried out at a temperature less than 140° C.;
- and (b) a denaturing distillation carried out at a temperature greater than or equal to 200° C.;
- wherein the purification step and the denaturing distillation step may be performed in either order;
- the temperature of the purification step and the denaturing distillation step is sufficient to cause the squalene to boil;
- and the denaturing distillation denatures and/or removes potential contaminant proteins and viruses, thereby rendering the squalene safe for human use as compared with non-denatured squalene;
- and
- (ii) preparing an oil-in-water emulsion adjuvant using the squalene prepared in step (i).
5 more elements
Dependencies are inferred from the claim text.