Process for the large scale production of fruit cells and treatment of diseases with such cells
AssigneeBio Harvest Ltd.
InventorsYoheved Hagay, Michal Abargel, Malkit Azachi, Yoav Roth, Rivka Yatuv
Life of this patent
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Abstract
The disclosure provides a method for treating, reducing, alleviating or preventing metabolic syndrome or metabolic malady, comprising administering a composition comprising a cell line callus culture of fruit cells grown in vitro. Further provided are fruit cells prepared according to a large scale process. The cell line callus culture of grape berry cells manufactured according to the process includes resveratrol in an amount of at least 1000 mg/kg powder.
Claims19
A composition in a form of a powder comprising a cell line callus culture of fruit cells, grape berry cells, grown in vitro in a large scale up process, whereby the cell line callus culture of grape berry cells is derived from one or more of grape-berry cross section, grape-berry skin, grape-berry flesh, grape seed, grape embryo of seeded or seedless cultivars or grape seed coat;
- wherein the cell line callus culture of grape berry cells includes glycoside resveratrol in the form of piceid in an amount of at least 1000 mg/kg powder, and wherein the large scale up process includes preparing a liquid suspension of the cell line callus culture and wherein the cell line callus culture is grown in the liquid suspension in at least one bioreactor.
A composition in a form of a powder comprising a cell line callus culture of fruit cells, grape berry cells, grown in vitro in a large scale up process, whereby the cell line callus culture of grape berry cells is derived from one or more of grape-berry cross section, grape-berry skin, grape-berry flesh, grape seed, grape embryo of seeded or seedless cultivars or grape seed coat;
- wherein the cell line callus culture of grape berry cells includes glycoside resveratrol in an amount of at least 1000 mg/kg powder, wherein the large scale up process comprises:
- growing fruit cells in a flask;
- inoculating the fruit cells from the flask into a first bioreactor;
- inoculating the fruit cells from the first bioreactor into a second bioreactor, wherein the process comprises a last bioreactor, and the second bioreactor is the last bioreactor or an intermediate bioreactor and wherein at least one of the first and the second bioreactor is disposable;
- and
- harvesting the fruit cells from the last bioreactor;
- wherein the fruit cells harvested from the last bioreactor are dried.
Dependencies are inferred from the claim text.