Therapeutic method using cardiac tissue-derived pluripotent stem cells
AssigneeKyoto University
InventorsHidemasa Oh, Kento Tateishi, Hiroaki Matsubara
Life of this patent
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Abstract
An object of the present invention is to provide a stem cell applicable to regenerative therapeutic method, and to provide a technique to carry out regenerative therapy using the cell. A collected cardiac tissue fragment is enzymatically treated to prepare a cell suspension. Then using the cell suspension, following steps are carried out: (1) separation of cells by the density gradient method, (2) suspension-culture in a culture medium containing fibroblast growth factor and epidermal growth factor and (3) selection and separation of cells forming a floating sphere to obtain pluripotent stem cells. Thus-obtained pluripotent stem cells are used to carry out regenerative therapy.
Claims18
A method for treating a cardiac tissue or organ in need of repair and/or regeneration in a patient comprising:
- transplanting a therapeutically effective amount of an enriched cell population of isolated human cardiac tissue-derived pluripotent stem cells into the cardiac tissue or organ in need of repair and/or regeneration in the patient,
- wherein the pluripotent stem cells are at least c-kit-negative, CD34-negative, CD105-positive, and CD90-positive, and have the capability of differentiating into one or more species of cells selected from the group consisting of cardiac myocyte, smooth myocyte, and vascular endothelial cell.
A method for treating a cardiac tissue or organ in need of repair and/or regeneration in a patient, comprising:
- transplanting a therapeutically effective amount of a composition comprising an enriched cell population of isolated human cardiac tissue-derived pluripotent stem cells and a pharmaceutically acceptable carrier into the cardiac tissue or organ in need of repair and/or regeneration in the patient,
- wherein the pluripotent stem cells are at least c-kit-negative, CD34-negative, CD105-positive, and CD90-positive, and have the capability of differentiating into one or more species of cells selected from the group consisting of cardiac myocyte, smooth myocyte, and vascular endothelial cell.
A method for treating a cardiac tissue or organ that is damaged, dysfunctional, and/or hypofunctional in a patient comprising:
- transplanting a therapeutically effective amount of an enriched cell population of isolated human cardiac tissue-derived pluripotent stem cells into the cardiac tissue or organ that is damaged, dysfunctional, and/or hypofunctional in the patient,
- wherein the pluripotent stem cells are at least c-kit-negative, CD34-negative, CD105-positive, and CD90-positive, and have the capability of differentiating into one or more species of cells selected from the group consisting of cardiac myocyte, smooth myocyte, and vascular endothelial cell.
Dependencies are inferred from the claim text.