Adaptively optimized biological components for biohybrid devices
OwnerJeffrey T. LaBelle, Vincent B. Pizziconiinventors
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Abstract
Embodiments of apparatus, compositions, methods, systems, and articles of manufacture are disclosed relating to the optimization and production of biological components for use in biohybrid photosensitive devices and systems and other applications. In some embodiments, biologically derived components are disclosed having properties and/or characteristics that are optimized for applications of interest relative to corresponding components derived from naturally occurring organisms. In some embodiments, properties and/or characteristics of biological components are optimized by subjecting organisms and/or populations thereof to forced adaptation.
Claims10
A method of producing a force-adapted population comprising organisms expressing force-adaptively optimized extractable biological components for a biohybrid device, the method comprising:
- (a) providing a physically segregated population of organisms expressing an extractable biological component having a characteristic desired to be optimized for enhanced biohybrid device performance;
- (b) physically segregating the population into a plurality of sub-populations;
- (c) selecting one or more environmental variables each representing the value of a controllable environmental condition;
- (d) evaluating a measure of the characteristic desired to be optimized in each sub-population after subjecting each sub-population during growth to controllable environmental conditions at pre-specified values of each of the selected environmental variables, each sub-population being subjected to a different selection of such values, wherein evaluating a measure of the characteristic desired to be optimized comprises measuring or observing at least one physical or chemical property relating to the characteristic;
- (e) determining from the evaluation a relation between the measure of the characteristic desired to be optimized and the values of each of the selected environmental variables corresponding thereto;
- (f) from the relation, determining values of the selected environmental variables corresponding to a desired value of a measure of a characteristic desired to be optimized;
- (g) growing a segregated population of the organisms while subjecting the population to controllable environmental conditions at the values of the environmental variables so determined, to produce at least one force-adapted organism wherein the characteristic desired to be optimized is altered in comparison to naturally occurring organisms of the same species;
- (h) extracting from the at least one force-adapted organism a plurality of extractable biological components each comprising a light antenna structure, and
- (i) disposing the extractable biological components in an apparatus wherein the extractable biological components are constrained to a substrate wherein the apparatus is configured to permit exposure of the light antenna structures to light.
Dependencies are inferred from the claim text.