Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high throughput screening

A pseudotyped lentiviral vector particle comprising a fusion protein (F) and a hemagglutinin (H) protein of a morbillivirus, wherein the cytoplasmic portions of the F and H proteins are truncated by deletion of amino acid residues from said cytoplasmic portions and

• wherein the truncated cytoplasmic portion of the F protein, located at the C-terminal end of the protein, comprises at least 1 positively charged amino acid residue and no more than 9 consecutive amino acid residues as counted from the N-terminal end of the cytoplasmic portion of the F protein and
• wherein the truncated cytoplasmic portion of the H protein comprises at least 9 and no more than 12 consecutive amino acid residues as counted from the C-terminal end of the cytoplasmic portion of the H protein plus an additional methinonine at the N-terminus, and
• wherein the pseudotyped lentiviral vector particle effects an enhanced transduction efficiency as compared with a lentiviral vector particle pseudotyped with wild-type F and H proteins, and
• wherein the truncated H protein is selected from the group consisting of HcΔ21, HcΔ22, HcΔ23, and HcΔ24, and
• wherein the truncated H protein has a single chain antibody, a growth factor or a ligand to a cell surface marker at its ectodomain.