Back to results
US 9,862,958 B2Granted
Nucleotide sequence and a process thereof
AssigneeBiocon Limited
InventorsNagaraj Govindappa, Sankar Periyasamy, Shivakumar Madenahalli Channabasappa, Suma Sreenivas, Kedamath Nanjund Sastry
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a process of identification and isolation of sorbitol dehydrogenase promoter from Pichia pastoris. Further, the present disclosure also relates to expression of heterologous proteins under the control of Sorbitol dehydrogenase promoter in Pichia pastoris.
Claims8
A process of isolation of SEQ ID NO:
- 1, wherein said process comprising acts of:
- a) isolating genomic DNA from P. pastoris;
- b) amplifying a region comprising SEQ ID NO:
- 1 within the isolated genomic DNA, wherein the amplification of SEQ ID NO:
- 1 is carried out by forward primer set forth as SEQ ID NO:
- 2 and reverse primer set forth as SEQ ID NO:
- 3;
- and
- c) subjecting the amplified product to a conventional gel elution procedure to isolate said SEQ ID No.1.
6 more elements
A process of expressing a gene under regulation of SEQ ID NO:
- 1 in a medium comprising non-specific inducer, wherein said process comprising acts of:
- a) cloning said gene in an expression construct downstream to the SEQ ID NO:
- 1;
- b) transforming a host with the expression construct;
- and
- c) expressing the gene under the regulation of said SEQ ID NO:
- 1 in a medium comprising non-specific inducer for production of heterologous protein.
4 more elements
A vector comprising the nucleotide sequence set forth as SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.