Method for in-vitro preparation of double-layered virus-like particles of rotavirus
AssigneesXiamen University, Xiamen Innovax Biotech Co., Ltd.
InventorsShengxiang Ge, Tingdong Li, Qingshun Guo, Feihai Xu, Jun Zhang, Ningshao Xia
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Abstract
The invention relates to a method for preparing double-layered virus-like particles of rotavirus in vitro. The method comprises the following steps: purifying rotavirus VP6 proteins from a lysis supernatant, and in vitro assembling double-layered virus-like particles consisting of VP2 proteins and VP6 proteins, wherein the proteins and the virus-like particles can be used for preventing or reducing the clinical symptoms caused by rotavirus infection.
Claims24
A method for preparing a double-layered virus-like particle of rotavirus comprising rotavirus VP2 protein and VP6 protein, the method comprising the following steps of:
- a) expressing VP6 in a soluble form in a prokaryotic expression system, and purifying the VP6, wherein the purified VP6 protein retains its native conformation and is present in a form of trimer;
- and
- b) co-assembling the purified VP6 with VP2 extracellularly, and with VP2 in a non-particulate state, to form a double-layered particle 2/6-VLP.
Dependencies are inferred from the claim text.