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US 9,856,530 B2Granted
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims30
A method for nucleic acid preparation or analysis, comprising:
- (a) providing:
- (i) at least 1,000 gel beads;
- (ii) releasably attached to each of said at least 1,000 gel beads, at least 1,000 barcode molecules comprising identical barcode sequences that are distinct from barcode sequences of at least 1,000 barcode molecules releasably attached to any other gel bead of said at least 1,000 gel beads;
- and
- (iii) a plurality of cells each comprising a plurality of polynucleotide molecules;
- (b) generating a plurality of droplets, wherein at least 1,000 droplets of said plurality of droplets each comprise:
- (i) a single gel bead from said at least 1,000 gel beads;
- and
- (ii) a single cell from said plurality of cells;
- and
- (c) in each of said at least 1,000 droplets, using said plurality of polynucleotide molecules from said single cell and barcode molecules of said at least 1,000 barcode molecules from said single gel bead to generate a plurality of barcoded polynucleotide molecules,
- wherein said barcode molecules become detached from said gel bead.
9 more elements
Dependencies are inferred from the claim text.