Method for non-fluorescence higher harmonic generation ground state depletion super-resolution microscopy

A method for non-fluorescence higher harmonic generation ground state depletion ultra-resolution microscopy, comprising the steps of:

• providing an organic material unit, wherein the organic material unit comprises a plurality of molecules, each said molecule has a plurality of electrons, and each said electron has an energy band with energy of hv such that, when excited by hv, the electrons jump from a ground state to a singlet state and undergo inter-system crossing from the singlet state to a triplet state, with h being the Planck constant (equal to 6.626×10^−34) and v being a frequency expressed in hertz (Hz);
• focusing excitation light and ground state depletion light by collimating the excitation light, projected by a long-wavelength ultrafast pulse laser, and the ground state depletion light, projected by a short-wavelength continuous-wave laser;
• combining the collimated excitation light with the collimated ground state depletion light;
• and focusing the combined light onto a plurality of test positions of the organic material unit sequentially;
• generating a higher harmonic signal by irradiating and exciting the test positions of the organic material unit with the focused excitation light such that the electrons of the molecules at the test positions are excited and jump to the singlet state and the molecules induce the higher harmonic generation signal;
• performing ground state depletion by irradiating with the focused ground state depletion light, and thus depleting, the electrons at the test positions that are in the ground state such that the electrons are excited and jump to the singlet state and undergo inter-system crossing to the triplet state;
• and
• performing microscopic imaging by receiving the higher harmonic generation signal of the test positions of the organic material unit and generating an ultra-resolution microscopic image corresponding to the organic material unit, with a stimulated emission depletion (STED) system.