Method for monitoring cancer and/or inflammatory reaction based on RelB phosphorylation
AssigneesCentre National de la Recherche Scientifique (CNRS), Universite Paris Descartes, Institut National De La Sante Et De La Recherche Medicale (Inserm)
InventorsVeronique Baud, Katy Billot
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Abstract
The present Inventors demonstrated that the RelB subunit of NFκB plays a crucial role in promoting cell migration. More precisely, they identified that this pro-migratory activity is mediated by the activation of the NFκB pathway through RelB phosphorylation at serine 472. In a first aspect, the present invention proposes to monitor the activation of the NFκB pathway by following the phosphorylation status of said serine. Also, the present invention discloses methods and kits for prognosing the evolution of a disease involving cell migration in a subject—treated or not—suffering thereof, based on the detection of said RelB-S472 phosphorylation.
Claims11
An in vitro method, comprising:
- a) providing a sample;
- and
- b) detecting RelB (SEQ ID NO:
- 1) phosphorylated on serine 472 or a RelB-homologue having at least 80% identity to (SEQ ID NO:
- 1) and phosphorylated on a corresponding serine, in said sample.
An in vitro screening method comprising:
- a) providing a candidate molecule,
- b) incubating the candidate molecule with RelB (SEQ ID NO:
- 1) in the presence of a IkB kinase in phosphorylating conditions, and
- c) detecting whether phosphorylation of serine 472 of RelB (SEQ ID NO:
- 1) occurs.
Dependencies are inferred from the claim text.