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US 9,851,359 B2Granted
Ultra bright dimeric or polymeric dyes with spacing linker groups
AssigneesSony Corporation of America, Sony Corporation
InventorsTracy Matray, Sharat Singh
Life of this patent
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Abstract
Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I): or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M, m and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.
Claims27
A compound having the following structure (I):
- or a stereoisomer, salt or tautomer thereof, wherein:
- M is, at each occurrence, independently a fluorescent dye;
- L1 is at each occurrence, independently either:
- i) an optional alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene, heteroalkynylene or heteroatomic linker;
- or ii) a linker comprising a functional group capable of formation by reaction of two complementary reactive groups;
- L2 and L3 are, at each occurrence, independently an optional alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene, heteroalkynylene or heteroatomic linker;
- L4 is, at each occurrence, independently a polyethylene oxide linker;
- R1 is, at each occurrence, independently H, alkyl or alkoxy;
- R2 and R3 are each independently H, OH, SH, alkyl, alkoxy, alkylether, heteroalkyl, —OP(═Ra)(Rb)Rc, Q, or a protected form thereof, or L′;
- R4 is, at each occurrence, independently OH, SH, O−, S−, ORd or SRd;
- R5 is, at each occurrence, independently oxo, thioxo or absent;
- Ra is O or S;
- Rb is OH, SH, O−, S−, ORd or SRd;
- Rc is OH, SH, O−, S−, ORd, OL′, SRd, alkyl, alkoxy, heteroalkyl, heteroalkoxy, alkylether, alkoxyalkylether, phosphate, thiophosphate, phosphoalkyl, thiophosphoalkyl, phosphoalkylether or thiophosphoalkylether;
- Rd is a counter ion;
- Q is, at each occurrence, independently a moiety comprising a reactive group, or protected form thereof, capable of forming a covalent bond with an analyte molecule, a targeting moiety, a solid support or a complementary reactive group Q′;
- L′ is, at each occurrence, independently a linker comprising a covalent bond to Q, a linker comprising a covalent bond to a targeting moiety, a linker comprising a covalent bond to an analyte molecule, a linker comprising a covalent bond to a solid support, a linker comprising a covalent bond to a solid support residue, a linker comprising a covalent bond to a nucleoside or a linker comprising a covalent bond to a further compound of structure (I);
- m is, at each occurrence, independently an integer of zero or greater, provided that at least one occurrence of m is an integer of one or greater, such that the compound includes at least one L4;
- and
- n is an integer of one or greater.
17 more elements
Dependencies are inferred from the claim text.