Device and methods for detection of analytes including use of a colorimetric barcode

A method for determining an analyte, comprising:

• contacting a surface of a rolling substrate of a sample collection device with an article suspected of containing an analyte via a rolling or tapping motion such that a sample suspected of containing the analyte is collected on the surface of the rolling substrate;
• contacting the surface of the rolling substrate with a fluid carrier to transfer at least a portion of the sample to the fluid carrier to produce a fluid sample suspected of containing the analyte;
• introducing the fluid sample suspected of containing the analyte into a sample inlet of a sample analysis device comprising a plurality of channels constructed and arranged to receive the fluid sample;
• treating the fluid sample with a plurality of solvents provided from a corresponding plurality of solvent chambers disposed within the sample analysis device, wherein the plurality of solvent chambers are in fluid communication with the sample inlet;
• simultaneously introducing portions of the fluid sample into each of a set of the plurality of channels, each of the set of channels comprising a different chemical reagent capable of generating a luminescent and/or colorimetric signal upon interaction with a specific analyte, wherein the analyte, if present in the fluid sample, interacts with one or more of the chemical reagents to produce a plurality of luminescent and/or colorimetric signals in at least some of the set of channels, wherein the plurality of channels are in fluid communication with the sample inlet;
• and
• determining the presence and/or identity of the analyte based on the plurality of luminescent and/or colorimetric signals.

A kit for determining an analyte, comprising:

• a sample collection device, comprising:
• a first region comprising a fluid container containing a fluid carrier;
• a second region comprising a surface constructed and arranged to contact an article suspected of containing the analyte and to receive fluid from the fluid container;
• a sample outlet in fluid communication with the first and second regions;
• and
• a housing containing the first region, the second region, and the sample outlet;
• and
• a sample analysis device, comprising:
• at least one sample inlet constructed and arranged to receive a sample suspected of containing an analyte from the sample outlet of the sample collection device;
• a plurality of solvent chambers disposed within the sample analysis device and providing a plurality of different solvents for dissolving the sample, wherein the plurality of solvent chambers are in fluid communication with the at least one sample inlet;
• and
• a channel in fluid communication with the at least one sample inlet and configured to receive the dissolved sample, the channel comprising a chemical reagent capable of generating a luminescent or colorimetric signal.