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US 9,850,545 B2Granted
Multi-primer assay for Mycoplasma detection
AssigneeLife Technologies Corporation
InventorsOlga Petrauskene, Pius Brzoska, Somaya Bit, Jen-Kuei Liu, Robert Tebbs, Manohar Furtado
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Abstract
Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.
Claims19
A multi-primer assay for detecting Mycoplasma in a sample, comprising:
- a.) contacting the sample with the plurality of oligonucleotide primers selected from the group consisting of SEQ ID NOs:1-144 in a single vessel;
- b.) performing a multi-primer amplification reaction in the vessel in the presence of a nucleic acid dye or a dye probe, wherein each of the plurality of oligonucleotide primers is present for participation in amplifying a target nucleic acid in the sample to produce an amplification product comprising the nucleic acid dye or the dye probe;
- and
- c.) detecting a signal from the nucleic acid dye or the dye probe of the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample;
- wherein the assay is capable of detecting more than 90 Mycoplasma species or related microorganisms Acholeplama laidlwaii and Spiroplasma citri.
2 more elements
A method for detecting Mycoplasma comprising:
- a.) extracting nucleic acid from a sample to be tested for Mycoplasma;
- b.) combining in a single vessel a plurality of oligonucleotide primers and the nucleic acid from the sample;
- c.) contacting the nucleic acid from the sample with the plurality of oligonucleotide primers in the vessel, said oligonucleotide primers selected from the group consisting of SEQ ID NOS:1-144;
- d.) performing a multi-primer amplification reaction in the vessel, wherein each of the plurality of oligonucleotide primers is present for participation in amplifying the sample to produce an amplification product;
- and
- e.) detecting the amplification product, wherein the presence of the amplification product indicates Mycoplasma in the sample;
- wherein the assay can detect more than 90 Mycoplasma species or related microorganisms Acholeplama laidlwaii and Spiroplasma citri.
4 more elements
Dependencies are inferred from the claim text.