Method for detecting lung cancer using lung cancer-specific methylation marker gene

A method of detecting methylation of CpG of 5′ UTR or exon 1 region of PCDHGA12, said method comprising:

• (a) obtaining a biological sample comprising DNA from lung cells from a patient;
• (b) obtaining a biological sample comprising DNA from normal lung cells from healthy controls;
• (c) treating the DNA from lung cells from the patient and normal lung cells with a bisulfite;
• (d) determining the level of methylation in the 5′ UTR or exon 1 region of PCDHGA12 treated with a bisulfite in the sample from a patient by identifying CpG islands in the 5′ UTR or exon 1 region of PCDHGA12 treated with the bisulfite and using primer(s) for amplifying a methylated fragment comprising the CpG of 5′ UTR or exon 1 region of PCDHGA12 treated with a bisulfite;
• and
• (e) determining the level of methylation in the 5′ UTR or exon 1 region of PCDHGA12 treated with a bisulfite in normal lung cells by identifying CpG islands in the 5′ UTR or exon 1 region of PCDHGA12 treated with the bisulfite and using primer(s) for amplifying a methylated fragment comprising the CpG of 5′ UTR or exon 1 region of PCDHGA12 treated with a bisulfite,
• wherein determining the level of methylation is performed using a primer pair that is:
• i) selected from the group consisting of SEQ ID NO. 5 and SEQ ID NO. 6, SEQ ID NO. 7 and SEQ ID NO. 8, and SEQ ID NO. 9 and SEQ ID NO. 10;
• ii) selected from the group consisting of SEQ ID NO. 11 to SEQ ID NO. 13, SEQ ID NO. 14 to SEQ ID NO. 16, and SEQ ID NO. 17 to SEQ ID NO. 19;
• or
• iii) a PCR primer pair of SEQ ID NO. 20 and SEQ ID NO. 21.