Automatic real-time PCR system for the various analysis of biological sample

An automatic real-time quantitative amplification system which can perform analysis of various biological samples, comprising:

• a deck loading a multi-well plate for treating the biological sample, which purifies a target nucleic acid in a target substance contained in the biological sample, cultures the target substance contained in the biological sample and then purifies the target nucleic acid, or purifies a binding target nucleic acid bound with a target antigen contained in the biological sample by an antigen-antibody reaction, and a multi-well plate for PCR, in which a reaction mixture for real-time quantitative PCR is injected;
• an automatic purification and reaction preparing device which automatically purifies the target nucleic acid or the cultured target nucleic acid from the biological sample and dispenses the purified target nucleic acid or the cultured and purified target nucleic acid to the multi-well plate for PCR, and then mixes the dispensed target nucleic acid with a reagent for real-time quantitative PCR, or which automatically purifies the binding target nucleic acid bound with the target antigen contained in the biological sample by the antigen-antibody reaction, and dispenses the purified binding target nucleic acid to the multi-well plate for PCR, and then mixes the dispensed binding target nucleic acid with the reagent for real-time quantitative PCR;
• an automatic deck storing and moving device including:
• a storing case which has a door for taking in or out the deck and of which an internal portion can be maintained at predetermined temperature,
• a stacking rack lifting unit which moves a stacking unit in an up and down direction,
• a plurality of racks arranged in the up and down direction in the stacking unit so that the plurality of racks move in the up and down direction when the sacking unit moves in the up and down direction, each of the plurality of racks configured to receive the deck, and
• a deck transferring unit movable in a left and right direction of the deck and transferring the deck loaded in the stacking rack to the automatic purification and reaction preparing device,
• a sealing device which seals an upper surface of the multi-well plate for PCR, in which the purified target nucleic acid, the cultured and purified target nucleic acid or the purified binding target nucleic acid is dispensed;
• a centrifugal separator which applies centripetal force to the multi-well plate for PCR so that a substance remained on a side wall of each well formed in the multi-well plate for PCR is separated and then moved to a bottom surface of the each well;
• a real-time quantitative amplification device which amplifies the target substance in the multi-well plate for PCR;
• and
• a moving device for multi-well plate for PCR, which moves the multi-well plate for PCR, in which the purified target nucleic acid, the cultured and purified target nucleic acid or the purified binding target nucleic acid is dispensed, to the sealing device, and moves the multi-well plate for PCR sealed by the sealing device to the centrifugal separator, and also moves the multi-well plate for PCR, to which the centripetal force is applied by the centrifugal separator, to the real-time quantitative amplification device,
• wherein the automatic purification and reaction preparing device comprises:
• a syringe block formed with a plurality of first mounting portions to which a plurality of pipettes P for sucking and discharging a fluid substance is removably mounted;
• and
• a syringe block moving unit which moves the syringe block so that the plurality of pipettes P mounted in the plurality of first mounting portions is located just above each of the multi-well plate for treating the biological sample and the multi-well plate for PCR.