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AssigneeANP Technologies, Inc.
InventorsYli Remo Vallejo, Jr., David Milunic, Ray Yin, Renee Street, De Chen
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A device and method for the rapid on-site detection of inhibitors of enzymes, such as acetylcholinesterase, is described wherein the device contains all reagents added to a sample pad containing dried releasable enzyme creating a reaction mixture wherein inhibitor deactivates the enzyme, while said reaction mixture travels via a longitudinal membrane to a distal porous pad containing a substrate for the enzyme. The reaction of the enzyme and the substrate results in a product that can generate a measurable signal such as color, fluorescence or luminescence to serve as a reporter. Signal that is generated at this reaction zone is inversely proportional to inhibitor concentration in the test sample. A device containing two such strips, one for a test sample, the other for a negative control fluid as an onboard comparator is described. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter.
A device for detecting an inhibitor of an enzyme comprising a longitudinal test strip enabling flow of fluid from one end to the other, comprising two pads of porous matrices on either end of said test strip and a fluid transfer membrane between the pads along the length of said test strip in liquid communication with each pad, one of said two pads comprising an upstream pad comprising a sample pad comprising releasably dried said enzyme, and second of said two pads comprising a downstream substrate pad comprising an immobilized substrate of said enzyme, wherein said substrate comprises a reporter which, on reaction with said enzyme, produces a signal in said device detectable visually or with a portable device, and said inhibitor reacts with said enzyme to form an inactivated enzyme that does not react with said substrate;
Dependencies are inferred from the claim text.