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US 9,850,515 B2Granted
Affinity-based partition assay for detection of target molecules
AssigneeBio-Rad Laboratories, Inc.
InventorsAdam M. McCoy, Svilen Tzonev, Frank Hsiung
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Abstract
The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.
Claims14
A method of detecting a target molecule in a sample, the method comprising:
- incubating said sample in a mixture with at least a first probe linked to a first label and a second probe linked to a second label, wherein the first and second probes specifically bind the same target molecule, if present;
- partitioning the mixture into two or more partitions, wherein on average the partitions have fewer than two probes;
- and
- detecting the presence of the first label and the second label in at least one same partition, thereby detecting the target molecule in the sample.
1 more element
A method of detecting a target molecule in a sample, wherein the target molecule is linked to, or is capable of generating, a detectable molecule, the method comprising:
- incubating the sample in a mixture with a first probe linked to a first label, wherein the first probe specifically binds the target molecule, if present;
- partitioning the mixture into two or more partitions, wherein on average the partitions have fewer than one probe;
- and
- detecting the presence of the first label and the detectable molecule in at least one same partition, thereby detecting the target molecule in the sample.
1 more element
Dependencies are inferred from the claim text.