Methods and compositions for preventing norleucine misincorporation into proteins
AssigneeGenentech, Inc.
InventorsMichael W. Laird, Karthik Veeravalli
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Abstract
The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.
Claims3
An E. coli host cell comprising a mutant metA allele, wherein the mutant metA allele comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, and SEQ ID NO:26, wherein the E. coli host cell further comprises nucleic acid encoding an anti-VEGF antibody or an anti-VEGF antibody fragment.
Dependencies are inferred from the claim text.