US 9,850,510 B2Granted

Method of producing L-amino acids

AssigneeCj Cheiljedang Corporation

InventorsJun Ok Moon, Sang Jo Lim, Do Hyun Kwon, Kwang Ho Lee, Hyun Won Bae

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Abstract

Provided is a method of producing L-amino acids by using a recombinant coryneform microorganism in which the expression of a target gene is weakened by using a gene transcription inhibition method.

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Claims3

Dependent claims

A method of producing L-amino acids, the method comprising

1) culturing a recombinant coryneform microorganism capable of producing L-amino acids in a medium, wherein the recombinant coryneform microorganism is transformed by inserting an acetate-inducible promoter downstream of a stop codon of a target gene in a chromosome such that transcription from the acetate-inducible promoter occurs in a direction opposite to the direction of the target gene transcription;
and
2) adding acetate during the culturing to cause a transcription from the acetate-inducible promoter in a direction opposite to the direction of the target gene transcription so that RNA polymerase complexes conflict with each other to weaken the expression of the target gene, and to strengthen the L-amino acid production capability of the recombinant coryneform microorganism,
wherein the target gene is at least one gene selected from the group consisting of a gene encoding a pyruvate dehydrogenase subunit E1, a gene encoding homoserine dehydrogenase, a gene encoding a UDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate ligase gene, and a gene encoding dihydrodipicolinate synthase,
if the target gene is at least one gene selected from the group consisting of a gene encoding a pyruvate dehydrogenase subunit E1, a gene encoding homoserine dehydrogenase, and a gene encoding a UDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate ligase gene, the 1-amino acid is L-lysine, or
if the target gene is at least one gene selected from the group consisting of a gene encoding a pyruvate dehydrogenase subunit E1 and a gene encoding dihydrodipicolinate synthase, the 1-amino acid is L-threonine.

3 more elements

Dependencies are inferred from the claim text.

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Abstract

Provided is a method of producing L-amino acids by using a recombinant coryneform microorganism in which the expression of a target gene is weakened by using a gene transcription inhibition method.

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Claims3

Dependent claims

A method of producing L-amino acids, the method comprising

1) culturing a recombinant coryneform microorganism capable of producing L-amino acids in a medium, wherein the recombinant coryneform microorganism is transformed by inserting an acetate-inducible promoter downstream of a stop codon of a target gene in a chromosome such that transcription from the acetate-inducible promoter occurs in a direction opposite to the direction of the target gene transcription;
and
2) adding acetate during the culturing to cause a transcription from the acetate-inducible promoter in a direction opposite to the direction of the target gene transcription so that RNA polymerase complexes conflict with each other to weaken the expression of the target gene, and to strengthen the L-amino acid production capability of the recombinant coryneform microorganism,
wherein the target gene is at least one gene selected from the group consisting of a gene encoding a pyruvate dehydrogenase subunit E1, a gene encoding homoserine dehydrogenase, a gene encoding a UDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate ligase gene, and a gene encoding dihydrodipicolinate synthase,
if the target gene is at least one gene selected from the group consisting of a gene encoding a pyruvate dehydrogenase subunit E1, a gene encoding homoserine dehydrogenase, and a gene encoding a UDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate ligase gene, the 1-amino acid is L-lysine, or
if the target gene is at least one gene selected from the group consisting of a gene encoding a pyruvate dehydrogenase subunit E1 and a gene encoding dihydrodipicolinate synthase, the 1-amino acid is L-threonine.

3 more elements

Dependencies are inferred from the claim text.

Description9,327 words

Summary445 words

#This application claims the benefit of Korean Patent Application No. 10-2013-0121090, filed on Oct. 11, 2013, and Korean Patent Application No. 10-2014-0091307, filed on Jul. 18, 2014, in the Korean Intellectual Property Office, the disclosures of which are incorporated herein in their entirety by reference.

#1. Field

#One or more embodiments of the present invention relate to a method of producing L-amino acids by using a gene transcription inhibition method.

Brief description of the drawings3 figures

#These and/or other aspects will become apparent and more readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings in which:

#FIG. 1 shows branch points of the amino acids biosynthesis process in a coryneform microorganism;

#FIG. 2A is a schematic diagram showing inhibition of aceE gene expression by inserting an aceA gene promoter between the stop codon and the transcription terminator upstream of the aceE gene in a direction opposite to the direction of the aceE gene transcription; and

Detailed description8,759 words

#Reference will now be made in detail to embodiments, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout. In this regard, the present embodiments may have different forms and should not be construed as being limited to the descriptions set forth herein. Accordingly, the embodiments are merely described below, by referring to the figures, to explain aspects of the present description. Expressions such as “at least one of,” when preceding a list of elements, modify the entire list of elements and do not modify the individual elements of the list.

#Hereinafter, the present invention is described in detail.

#One embodiment of the present invention provides a method of producing L-amino acids, the method including

Abstract

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Abstract

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Description9,327 words

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Classification13 codes

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Description9,327 words