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US 9,850,506 B2Granted
Modified microorganism for improved production of fine chemicals on sucrose
AssigneeBASF SE
InventorsJoanna Martyna Krawczyk, Stefan Haefner, Hartwig Schröder, Esther Dantas Costa, Oskar Zelder, Gregory Von Abendroth
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Abstract
The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the fruA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.
Claims7
A method of producing succinic acid comprising
- I) cultivating a modified microorganism in a culture medium comprising sucrose as an assimilable carbon source to allow the modified microorganism to produce succinic acid, thereby obtaining a fermentation broth comprising succinic acid, wherein the modified microorganism is a modified bacterial cell of the genus Basfia in which the fruA-gene or at least a part thereof has been deleted;
- II) recovering succinic acid from the fermentation broth obtained in process step I);
- wherein the assimilable carbon source is not a mixture of glycerol and sucrose and wherein at least 95 wt.-% of the assimilable carbon source, based on the total weight of the assimilable carbon source with the exception of carbon dioxide, is sucrose.
Dependencies are inferred from the claim text.