Biological methods for preparing a fatty dicarboxylic acid
AssigneeVerdezyne, Inc.
InventorsTom Beardslee, Alex Hutagalung, Stephen Picataggio
Life of this patent
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Abstract
The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids.
Claims15
A genetically modified yeast comprising (i) an acyl-CoA oxidase polypeptide, wherein the acyl-CoA oxidase polypeptide comprises a genetic modification that alters the enzyme activity of native acyl-CoA oxidase polypeptide;
- or an acyl-CoA dehydrogenase polypeptide, wherein the acyl-CoA dehydrogenase polypeptide comprises a genetic modification that alters the enzyme activity of native acyl-CoA dehydrogenase polypeptide;
- and (ii) an enoyl CoA isomerase polypeptide, wherein the enoyl CoA isomerase polypeptide comprises a genetic modification that alters the enzyme activity of native enoyl CoA isomerase polypeptide, wherein the yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil;
- and wherein the genetic modification is performed by:
- (i) adding a heterologous polynucleotide that encodes said polypeptide;
- (ii) adding a regulatory sequence that regulates the expression of said polypeptide;
- (iii) disrupting the polynucleotide that encodes said polypeptide;
- (iv) deleting the regulatory sequence that regulates the expression of said polypeptide;
- (v) deleting the coding sequence that encodes said polypeptide;
- or
- (vi) any combination thereof.
A method for producing a diacid, comprising:
- a) contacting a genetically modified yeast with a feedstock comprising a vegetable oil, wherein the genetically modified yeast comprises:
- (i) an acyl-CoA oxidase polypeptide, wherein the acyl-CoA oxidase polypeptide comprises a genetic modification that alters the enzyme activity of native acyl-CoA oxidase polypeptide;
- or an acyl-CoA dehydrogenase polypeptide, wherein the acyl-CoA dehydrogenase polypeptide comprises a genetic modification that alters the enzyme activity of native acyl-CoA dehydrogenase polypeptide;
- and
- (ii) an enoyl CoA isomerase polypeptide, wherein the enoyl CoA isomerase polypeptide comprises a genetic modification that alters the enzyme activity of native enoyl CoA isomerase polypeptide, wherein the yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil;
- and wherein the genetic modification is performed by:
- (i) adding a heterologous polynucleotide that encodes said polypeptide;
- (ii) adding a regulatory sequence that regulates the expression of said polypeptide;
- (iii) disrupting the polynucleotide that encodes said polypeptide;
- (iv) deleting the regulatory sequence that regulates the expression of said polypeptide;
- (v) deleting the coding sequence that encodes said polypeptide;
- or
- (vi) any combination thereof;
- and
- b) culturing the yeast under conditions in which diacid is produced from the feedstock.
Dependencies are inferred from the claim text.