Method for purifying active GLA-domain coagulation proteins

A method for purifying biologically active GLA-domain coagulation proteins, comprising the following steps:

• a) bringing a sample which contains one or more biologically active GLA-domain coagulation proteins, and which contains biologically inactive molecules of one or more GLA-domain protein(s), into contact with an affinity support having thereon immobilized nucleic aptamers which bind specifically to at least one biologically active GLA-domain coagulation protein, but not to biologically inactive molecules of said at least one GLA-domain coagulation protein, in order to form complexes between (i) said nucleic aptamers and (ii) said biologically active GLA-domain coagulation protein(s),
• b) releasing the biologically active GLA-domain coagulation protein(s) from the complexes formed in step a), and
• c) recovering said biologically active GLA-domain coagulation protein(s) in a purified form.