Method for delivering agents into cells using bacterial toxins
AssigneePresident and Fellows of Harvard College
InventorsR. John Collier, Bradley L. Pentelute
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Abstract
The invention provides compositions and methods for delivering a bioactive moiety comprising at least one non-natural component into a cell cytosol of a eukaryotic cell. The bioactive moiety is linked to an A component of a bacterial toxin, a functional wild-type or modified fragment thereof, or an A component surrogate or mimetic. For delivery, the cell is contacted with the linked bioactive moiety and a corresponding B component of the bacterial toxin or a functional fragment thereof.
Claims12
A fusion molecule comprising a bioactive moiety comprising at least one non-natural component and an A component of a bacterial toxin or an A component surrogate or a functional variant or fragment thereof, wherein the non-natural component is selected from a peptide or protein comprising one or more D-amino acids or a cyclic peptide.
Dependencies are inferred from the claim text.