Enzymatic synthesis of L-nucleic acids
AssigneeNOXXON Pharma AG
InventorsAndreas Pech, Ralf David, Florian Jarosch, Michael Jahnz, Sven Klussmann
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Abstract
The invention relates to a method of reacting one or more L-nucleotides with a first L-nucleic acid in the presence of a D-enzyme that adds the one or more L-nucleotides to the 3′ end of the first L-nucleic acid.
Claims13
A method for adding one or more L-nucleotides to the 3′ end of a first L-nucleic acid comprising reacting the one or more L-nucleotides with the first L-nucleic acid in the presence of a protein comprising an enzymatic activity of adding one or more L-nucleotides to the 3′ end of an L-nucleic acid, wherein the protein consists of African Swine Fever Virus polymerase X or polymerase DPO4, with the proviso that every amino acid of said polymerase X and polymerase DPO4 is substituted with the corresponding D-amino acid.
A method for amplifying a target L-nucleic acid comprising reacting said target L-nucleic acid with an L-nucleotide and a protein comprising an enzymatic activity of amplifying a target L-nucleic acid, wherein the protein consists of African Swine Fever Virus polymerase X or polymerase DPO4, with the proviso that every amino acid of said polymerase X and polymerase DPO4 is substituted with the corresponding D-amino acid.
A protein that adds one or more L-nucleotides to the 3′ end of a first L-nucleic acid, wherein the protein consists of African Swine Fever Virus polymerase X or polymerase DPO4, with the proviso that every amino acid of said polymerase X and polymerase DPO4 is substituted with the corresponding D-amino acid.
A polymerase of African Swine Fever Virus polymerase X or polymerase DPO4, with the proviso that every amino acid of said polymerase X and polymerase DPO4 is substituted with the corresponding D-amino acid.
Dependencies are inferred from the claim text.