Back to results
US 9,850,465 B2Granted
Generation of thymic epithelial progenitor cells in vitro
AssigneeThe Regents of the University of California
InventorsAudrey Parent, Matthias Hebrok, Mark Stuart Anderson
Life of this patent
Estimating the term…
Abstract
Methods for generating thymic epithelial progenitor (TEP) cells from pluripotent stem (PS) cells in vitro are provided. Compositions and systems of cell populations of TEP cells as well as cells formed during different stages of differentiation of PS cells into TEP cells are also disclosed. The methods, isolated in vitro cell populations, compositions, and systems disclosed provide functional TEP cells that mature into thymic epithelial cells in vivo.
Claims19
A method for generating thymic epithelial progenitor (TEP) cells, the method comprising:
- culturing definitive endodermal (DE) cells, obtained from pluripotent stem cells, in a medium comprising an activator of retinoic acid receptor, an activator of bone morphogenetic protein (BMP) signaling, and an inhibitor of transforming growth factor-β (TGF-β) signaling to produce anterior foregut endodermal (AFE) cells;
- culturing the AFE cells in a medium comprising an activator of retinoic acid receptor, an activator of bone morphogenetic protein (BMP) signaling, and an inhibitor of transforming growth factor-β (TGF-β) signaling to produce ventral pharyngeal endodermal (VPE) cells;
- and
- culturing the VPE cells in a medium comprising an activator of retinoic acid receptor and an activator of bone morphogenetic protein (BMP) signaling to produce the TEP cells.
1 more element
Dependencies are inferred from the claim text.