Peptide combination
AssigneeGKL-Biotec AG
InventorGuenther Klett-Loch
Life of this patent
Estimating the term…
Abstract
The invention relates to a peptide combination characterized by peptides each having the same sequence length (SEQL), that can be produced from a mixture (A) comprising a number x of amino acids having a protected acid group or a number z of peptides having an acid group protected by means of a protecting group and an activated amino group, wherein the amino acids are present in the mixture (A) in particular adjustable molar ratios, and a mixture (B) comprising a number y of amino acids having an amino group protected by means of a protecting group, wherein the amino acid molar ratios of the mixture (B) are equal to the amino acid molar ratios of the mixture (A), and wherein the number x=y.
Claims7
A combination of peptides comprising tetrapeptides, wherein the amino acids of said combination of peptides consist of a number x of different amino acids, wherein x is selected from 11 up to and including 18, and wherein said combination is produced by:
- a) combining:
- i) a first mixture (A) comprising said number x of-different amino acids having a protected acid group, and wherein the amino acids comprising at least aspartate, glutamate, proline, and glycine, wherein the amino acids are present in the first mixture (A) in certain, adjustable mole ratios, and wherein aspartate is present in 8.33 mol-%, glutamate is present in 9.53 mol-%, proline is present in 15.18 mol-%, and glycine is present in 37.10 mol-%;
- and,
- ii) a first mixture (B) comprising said number x of amino acids having amino groups protected by means of protective groups, wherein the amino acid mole ratios in the first mixture (B) are equal to the amino acid mole ratios in the first mixture (A);
- b) activating the protected amino functional groups of the resulting combination of peptides comprising dipeptides by cleavage of the amino protective groups, thereby producing a combination of peptides comprising dipeptides having protected acid groups and having activated amino groups;
- c) combining:
- i) a second mixture (A) comprising said combination of peptides comprising dipeptides having protected acid groups and having activated amino groups;
- and,
- ii) a second mixture (B) comprising said number x of amino acids having amino groups protected by means of protective groups, wherein the amino acid mole ratios in the second mixture (B) are equal to the amino acid mole ratios in the second mixture (A);
- d) activating the protected amino functional groups of the resulting combination of peptides comprising tripeptides by cleavage of the amino protective groups, thereby producing a combination of peptides comprising tripeptides having protected acid groups and having activated amino groups;
- e) combining:
- i) a third mixture (A) comprising said combination of peptides comprising tripeptides having protected acid groups and having activated amino groups;
- and,
- ii) a third mixture (B) comprising said number x of amino acids having amino groups protected by means of protective groups, wherein the amino acid mole ratios in the third mixture (B) are equal to the amino acid mole ratios in the third mixture (A).
A method for synthesizing a combination of peptides comprising tetrapeptides, wherein the amino acids of said combination of peptides consist of a number x of different amino acids, wherein x is selected from 11 up to and including 18, said method comprising:
- a) combining:
- i) a first mixture (A) comprising said number x of different amino acids having a protected acid group, and wherein the amino acids comprising at least aspartate, glutamate, proline, and glycine, wherein the amino acids are present in the first mixture (A) in certain, adjustable mole ratios, and wherein aspartate is present in 8.33 mol-%, glutamate is present in 9.53 mol-%, proline is present in 15.18 mol-%, and glycine is present in 37.10 mol-%;
- and,
- ii) a first mixture (B) comprising said number x of amino acids having amino groups protected by means of protective groups, wherein the amino acid mole ratios in the first mixture (B) are equal to the amino acid mole ratios in the first mixture (A);
- b) activating the protected amino functional groups of the resulting combination of peptides comprising dipeptides by cleavage of the amino protective groups, thereby producing a combination of peptides comprising dipeptides having protected acid groups and having activated amino groups;
- c) combining:
- i) a second mixture (A) comprising said combination of peptides comprising dipeptides having protected acid groups and having activated amino groups;
- and,
- ii) a second mixture (B) comprising said number x of amino acids having amino groups protected by means of protective groups, wherein the amino acid mole ratios in the second mixture (B) are equal to the amino acid mole ratios in the second mixture (A);
- d) activating the protected amino functional groups of the resulting combination of peptides comprising tripeptides by cleavage of the amino protective groups, thereby producing a combination of peptides comprising tripeptides having protected acid groups and having activated amino groups;
- e) combining:
- i) a third mixture (A) comprising said combination of peptides comprising tripeptides having protected acid groups and having activated amino groups;
- and,
- ii) a third mixture (B) comprising said number x of amino acids having amino groups protected by means of protective groups, wherein the amino acid mole ratios in the third mixture (B) are equal to the amino acid mole ratios in the third mixture (A).
Dependencies are inferred from the claim text.