Composition and methods of use
OwnerJanet Angelinventor
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Abstract
Provided herein are compositions and methods that can remove, metabolize, or degrade a hydrocarbon in an area that is contaminated by hydrocarbons. Methods for bioremediation of an area such as an area of land, a body of water, or a shoreline that are contaminated by a hydrocarbon, such as from a crude oil spill are also described. The compositions and methods described herein can be used on natural flora and fauna as well as manmade materials that are contaminated by a hydrocarbon.
Claims23
A method for promoting bioremediation in an area contaminated by a hydrocarbon selected from the group consisting of crude oil, refined oil, and petroleum products comprising:
- (a) providing an amount of a composition comprising
- (i) a microbial component comprising a consortium of microbes comprising the bacteria Bacillus licheniformis, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus pumilus, Pseudomonas putida, Pseudomonas fluororescens, Lactobacillus acidophilus, and L. Salivarius bifidum;
- (ii) an enzymatic component;
- (iii) an emulsifying component;
- and
- (iv) a nutritive component;
- (b) applying an amount of the composition to at least a portion of the area contaminated by the hydrocarbon, wherein applying is performed under condition that allow the composition to promote in situ bioremediation.
A method for cleaning hydrocarbons from a surface that is contaminated by hydrocarbons selected from the group consisting of crude oil, refined oil, and petroleum products comprising:
- (a) providing an amount of a composition comprising
- (i) a microbial component comprising a consortium of microbes comprising the bacteria Bacillus licheniformis, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus pumilus, Pseudomonas putida, Pseudomonas fluororescens, Lactobacillus acidophilus, and L. Salivarius bifidum;
- (ii) an enzymatic component;
- (iii) an emulsifying component;
- and
- (iv) a nutritive component;
- (b) applying an amount of the composition to at least a portion of the surface contaminated by hydrocarbons, wherein the applying is performed under conditions that allow for the hydrocarbons to be removed.
Dependencies are inferred from the claim text.