Pharmaceutical composition containing honeysuckle extract and antibiotics, pharmaceutical kit, and use of honeysuckle extract for preparation of drug

A pharmaceutical kit for preventing and/or treating a disease caused by a bacterium, comprising a honeysuckle extract containing iridoid compounds and an antibiotic which are placed separately;

• wherein the honeysuckle extract contains secologanic acid represented by the following structural formula (1):
• wherein, X1 and X2 each independently represent O, and R represents H;
• and
• wherein the honeysuckle extract contains 50 wt % or more of secologanic acid represented by formula (1),
• wherein preventing and/or treating a disease caused by a bacterium is achieved by reversing resistance of bacteria;
• wherein the antibiotic is ampicillin and/or erythromycin, and the antibiotic is greater in weight amount than that of the honeysuckle extract;
• and
• wherein the honeysuckle extract is prepared by a method comprising the following steps:
• (1) pulverizing honeysuckle (Lonicera japonica), and extracting the pulverized honeysuckle with water and/or C1-C6 alkyl alcohol aqueous solution containing not more than 95% alcohol by volume to obtain an extract;
• (2) concentrating the extract obtained in step (1) under normal or reduced pressure to obtain an extractum, or performing spray-drying on the extract obtained in step (1) to obtain a powder, and dissolving the extractum or powder with water, then carrying out precipitation or settlement with C1-C6 alkyl alcohol aqueous solution containing not more than 95% alcohol by volume to obtain a precipitate or a dissolving liquid concentrate;
• (3) isolating and purifying the precipitate or dissolving liquid concentrate obtained in step (2) by chromatography, and collecting eluent containing iridoid compounds, wherein the chromatography is selected from one or more of macroporous adsorption resin column chromatography, normal phase silica gel chromatography and reversed phase silica gel chromatography.