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US 9,848,615 B2Granted
Storage stable frozen lactic acid bacteria culture
AssigneeChr. Hansen A/S
InventorsHans Bisgaard-Frantzen, Børge Windel Kringelum, Inge Knap
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Abstract
A storage stable frozen lactic acid bacteria (LAB) culture that comprises LAB that are that are able to utilize sucrose, has a weight of at least 50 g frozen material and a content of viable bacteria of at least 109 colony forming units (CFU) per g frozen material.
Claims19
A frozen lactic acid bacteria culture comprising:
- a mixture of mesophilic lactic acid bacteria that (a) have an optimum growth temperature of about 30° C. and (b) are capable of utilizing sucrose, and
- from 0.5% to 80% w/w of at least one cryoprotective agent,
- wherein the frozen culture is not freeze-dried and has a weight of at least 50 grams and comprises at least 109 colony forming units of viable bacteria per gram.
A method for making a frozen lactic acid bacteria culture comprising:
- (i) adding at least one cryoprotective agent to a mixture of mesophilic lactic acid bacteria that (a) have an optimum growth temperature of about 30° C. and (b) are capable of utilizing sucrose;
- and then
- (ii) freezing the culture,
- wherein the frozen culture is not freeze-dried and has a weight of at least 50 grams and comprises (a) at least 109 colony forming units viable bacteria per gram and (b) from 0.5% to 80% w/w of the at least one cryoprotective agent.
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Dependencies are inferred from the claim text.